High-mobility group box protein-1 released by human-periodontal ligament cells modulates macrophage migration and activity in vitro:

Innate Immunity

MichaelWolf¹ 

关键词: HMGB1;    periodontal ligament cells;    macrophages;    in vitro;   

DOI  :  10.1177/1753425913505121

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Recent studies have demonstrated the interplay of human periodontal ligament cells (hPDLs) with immune cells, such as macrophages, during tissue repair. High-mobility group box protein-1 (HMGB1) is released into the extracellular milieu by damaged cells and functions as an alarmin to mediate the inflammatory host response. The present study addressed the role of HMGB1 released by hPDLs in the regulation of macrophage differentiation, migration and activity. The aim was to examine the inflammatory potential of HMGB1 itself and in combination with other mediators. The induction of sterile necrosis by thermal insult of hPDLs resulted in HMGB1 translocation from the nucleus to the cytoplasm and on to the extracellular space, as determined by immunocytochemistry/ELISA. Exposure of human macrophages to the conditioned PDL cell medium increased the expression of macrophage differentiation/activation markers CD14, CD23, CD64 and CD163. Chemotactic migration and osteoclastic differentiation of macrophages were als...

【 授权许可】

CC BY