Immunohistochemical expression of heat shock proteins in the mouse periodontal tissues due to orthodontic mechanical stress

European Journal of Medical Research

T Kawakami¹  K Yamada³  S Kurihara¹  K Nakano¹  R Muraoka² 

[1] Matsumoto Dental University Institute for Oral Science, Shiojiri, Japan;Department of Orthodontics, Matsumoto Dental University School of Dentistry, Shiojiri, Japan;Clinical Evaluation Unit, Matsumoto Dental University Graduate School of Oral Medicine, Shiojiri, Japan

关键词: orthodontic tooth movement;    mechanical stress;    periodontal ligament cells;    HSP27;    Heat shock protein;   

Others  :  829513
DOI  :  10.1186/2047-783X-15-11-475

 received in 2010-06-04, accepted in 2010-07-29,  发布年份 2010

【 摘 要 】

The histopathology of periodontal ligament of the mouse subjected to mechanical stress was studied. Immunohistochemical expressions of HSP27 and pHSP27 were examined. Experimental animals using the maxillary molars of ddY mouse by Waldo method were used in the study. A separator was inserted to induce mechanical stress. After 10 minutes, 20 minutes, 1 hour, 3 hours, 9 hours and 24 hours, the regional tissues were extracted, fixed in 4% paraformaldehyde and 0.05 M phosphate-buffered fixative solution. Paraffin sections were made for immunohistochemistry using HSP27 and p-HSP27. In the control group, the periodontal ligament fibroblasts expressed low HSP27 and p-HSP27. However, in the experimental group, periodontal ligament fibroblasts expressed HSP27 10 minutes after mechanical load application in the tension side. The strongest expression was detected 9 hours after inducing mechanical load. p-HSP27 was also expressed in a time-dependent manner though weaker than HSP27. The findings suggest that HSP27 and p-HSP27 were expressed for the maintenance of homeostasis of periodontal ligament by the activation of periodontal ligament fibroblasts on the tension side. It also suggests that these proteins act as molecular chaperones for osteoblast activation and maintenance of homeostasis.

【 授权许可】

   
2010 I. Holzapfel Publishers

【 图 表 】

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