Development of an electrochemical Limulus amebocyte lysate assay technique for portable and highly sensitive endotoxin sensor:

Innate Immunity

Kumi YInoue¹ 

关键词: Biosensor;    carbon electrode;    endotoxin assay;    lipopolysaccharide;    voltammetry;   

DOI  :  10.1177/1753425911410337

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Here, we report the development of an electrochemical detection method for endotoxin based on the Limulus amebocyte lysate (LAL) assay. A mixture of LAL reagent and endotoxin sample solution was incubated for 1 h. The endotoxin activated a cascade reaction of zymogens contained in the LAL to generate p-nitroaniline (pNA) which was then electrochemically detected by differential pulse voltammetry (DPV). The generated pNA gave a clear peak at –0.75 V vs. silver/silver chloride (Ag/AgCl), which increased with the concentration of endotoxin in the LAL assay solution. This DPV detection was performed using an electrode chip device fabricated from a diamond-like carbon-coated glass substrate. This chip device could detect as low as 10 endotoxin units l−1 at room temperature within 1 h. This novel electrochemical method for the detection of endotoxin appears promising for the development of compact, low-cost and easy-to-use sensors for on-site monitoring of potentially contaminated medical supplies, including di...

【 授权许可】

CC BY