Identification of putative innate immune related genes from a cell line of the mosquito Aedes albopictus following bacterial challenge:

Innate Immunity

RajnikantDixit¹ 

关键词: Aedes albopictus;    innate immunity;    subtractive hybridization;    mosquito;    ESTs;   

DOI  :  10.1177/1753425909350484

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

We report identification of putative innate immune related genes from a cell line of the mosquito Aedes albopictus challenged with heat-killed bacteria. Using a subtractive hybridization and sequencing approach, we analyzed a total 309 expressed sequence tags (ESTs) which clustered in 40 contigs. Thirty-five percent of genes yielded homology to known immune genes corresponding to antimicrobial peptides (AMPs), pathogen-associated molecular patterns, protease and immune signaling cascades. Interestingly, most of the genes have not been previously described from this mosquito and thus represent a class of novel immune genes. Further, 25% sequences did not match to any known species in the non-redundant databases, appear to be specific to the mosquito A. albopictus and merit further study.

【 授权许可】

CC BY