Increased Pathogen Identification in Vascular Graft Infections by the Combined Use of Tissue Cultures and 16S rRNA Gene Polymerase Chain Reaction

Frontiers in Medicine

Evelyne Ajdler-Schaeffler¹ 

关键词: 16S rRNA gene polymerase chain reaction;    vascular graft infection;    pathogen identification;    microbiological diagnosis;    tissue culture;   

DOI  :  10.3389/fmed.2018.00169

学科分类：医学（综合）

来源: Frontiers

【 摘 要 】

Background: Vascular graft infections (VGI) are difficult to diagnose and treat, and despite redo surgery combined with antimicrobial treatment, outcomes are often poor. VGI diagnosis is based on a combination of clinical, radiological, laboratory and microbiological criteria. However, as many of the VGI patients are already under antimicrobial treatment at the time of redo surgery, microbiological identification is often difficult and bacterial cultures often remain negative rendering targeted treatment impossible. We aimed to assess the benefit of 16S rRNA gene polymerase chain reaction (broad-range PCR) for better microbiological identification in patients with VGI.

【 授权许可】

CC BY