TLR2-stimulating contaminants in glycoconjugate fractions prepared from Bacteroides fragilis:

Innate Immunity

MasahitoHashimoto¹ 

关键词: Bacteroides fragilis;    capsular polysaccharide;    lipopolysaccharide;    lipoprotein;    Toll-like receptor;   

DOI  :  10.1177/1753425917714313

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Bacteroides fragilis is a member of the normal intestinal flora and is involved in host immunostimulation via TLR2. On the bacterial cell surface, glycoconjugates, such as LPS and capsular polysaccharide A (PSA), have been reported to participate in host immunostimulation via TLR2. Previously, we identified a TLR2-stimulating lipoprotein in B. fragilis cells. In this study, we demonstrated that TLR2-stimulating principal molecules in glycoconjugate fractions prepared from B. fragilis are contaminating proteinous molecules, which may also be lipoproteins. The glycoconjugate fractions were prepared by phenol–hot water extraction of B. fragilis wild type and PSA-deficient strains, followed by hydrophobic interaction chromatography. TLR2-stimilating activities of the fractions were not affected by PSA deficiency. By in-gel TLR2-stimulation assay, molecules in high-molecular-mass area, where capsular polysaccharides were migrated, were found not to stimulate TLR2, but those in the range of 15–40 kDa were activ...

【 授权许可】

CC BY