Identification of a TLR2-stimulating lipoprotein in Bacteroides fragilis JCM 11019 (NCTC 9343):

Innate Immunity

MasahitoHashimoto¹ 

关键词: Bacteroides fragilis;    lipoprotein;    mass spectra;    Toll-like receptor 2;    Triton X-114;   

DOI  :  10.1177/1753425912454179

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Bacteroides fragilis is found among the normal intestinal flora and is involved in host immunostimulation via TLR2. Its cell surface components, such as LPS and capsular polysaccharides, were reported to participate in host immunostimulation. In this study, we report on the existence of a lipoprotein that acts as a TLR2 stimulant in B. fragilis. The TLR2-stimulating lipoprotein was obtained using Triton X-114-water phase partitioning followed by preparative SDS-PAGE. Its N-terminal hydrophobic peptide, which was separated from a tryptic digest, was characterized as a triacylated lipopeptide, and the lipoprotein was identified as BF1333 by mass spectrometry of Asp-N-digested peptides. These results showed that the lipoprotein acts as a TLR2-stimulating component in B. fragilis.

【 授权许可】

CC BY