Effect of antimicrobial peptides from Apis mellifera hemolymph and its optimized version Api88 on biological activities of human monocytes and mast cells:

Innate Immunity

UlrikeKeitel¹ 

关键词: Apidaecin;    lipopolysaccharide;    TNF-α;    phagocytosis;    reactive oxygen species;    monocytes;    mast cells;   

DOI  :  10.1177/1753425912462045

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Apidaecin peptides are produced by the honeybee Apis mellifera as a major part of its non-specific defense system against infections. Having verified that the peptides apidaecin 1b and Api88—a designer peptide based on the native apidaecin 1b sequence—are highly active against Gram-negative bacteria, we studied their ability to modulate biological activities of human monocytes and mast cells (MC), two important cell types of the human innate immune system. We could show that both peptides are nontoxic and fairly resistant to degradation in cell culture medium containing 10% FBS. Among the peptides tested we found Api88 to inhibit LPS-induced TNF-α production in a concentration-dependent manner. Resting monocytes did not respond to Api88. Whilst Api88 neither induced migration nor affected the phagocytic activity of monocytes it partially inhibited the generation of reactive oxygen intermediates produced in response to LPS. In human MC, however, Api88 triggered degranulation and the mobilization of intrace...

【 授权许可】

CC BY