Innate Immunity | |
The influence of vitamin D on M1 and M2 macrophages in patients with Crohn's disease: | |
SergeDionne1  | |
关键词: Vitamin D; macrophages; Crohn’s disease; cytokines; innate immunity; | |
DOI : 10.1177/1753425917721965 | |
学科分类:生物科学(综合) | |
来源: Sage Journals | |
【 摘 要 】
Defective bacterial clearance by macrophages plays an important role in Crohn’s disease (CD). Phenotypes and functions of inflammatory M1 and anti-inflammatory M2 have not been studied in CD. Vitamin D supplementation reduces the severity of CD by unclear mechanisms. We studied macrophage characteristics in CD and controls and the effects of 1,25 vitamin D (1,25D). PBMC were isolated from CD patients and controls. M1 and M2 were generated by culturing of monocytes with GM-CSF and M-CSF, respectively. CD M1 and M2 showed normal phagocytosis and chemotaxis to CCL2 and fMLP. LPS-induced production of TNF-α, IL-12p40 and IL-10 was comparable between groups. Phagocytosis was unaltered with 1,25D; migration only increased marginally. M1 produced more IL-12p40 and TNF-α; IL-10 was greater in M2. 1,25D markedly decreased IL-12p40 by M1 and M2. 1,25D decreased TNF-α in CD M1; IL-10 levels were unaffected. M2 express F13A1, PTGS2, CD163, CXCL10, CD14 and MMP2, whereas TGF-β, CCL1 and CYP27B1 expression was higher i...
【 授权许可】
CC BY
【 预 览 】
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RO201901224020197ZK.pdf | 398KB | download |