期刊论文详细信息
SAGE Open Medicine
Detection of asymptomatic renal Leptospira infection in abattoir slaughtered cattle in southeastern Georgia, United States:
SreekumariRajeev1 
关键词: Leptospira;    cattle;    diagnosis;    public health;   
DOI  :  10.1177/2050312114544696
学科分类:医学(综合)
来源: Sage Journals
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【 摘 要 】

Objectives:Leptospirosis is one of the most widespread zoonotic infectious diseases affecting humans and animals. Several animal species, including cattle, can act as potential asymptomatic carriers facilitating zoonotic transmission of Leptospira. This study was conducted to assess the occurrence of asymptomatic renal Leptospira carriers among cattle slaughtered in southeastern Georgia, United States.Methods:A battery of diagnostic tests, including dark field microscopy, direct fluorescent antibody staining, polymerase chain reaction, and culture, were performed on a set of bovine kidneys (n = 37) collected from an abattoir in southeastern Georgia, United States. Virulence of a field isolate obtained from this study was tested in a hamster experimental model.Results:Motile spirochete-like structures were observed by dark field microscopy in 23 (59%) out of 37 kidney samples tested. In all, 29 samples (78%) were positive by direct fluorescent antibody staining. Only 11 (29.7%) samples by polymerase chain ...

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