Isolation of leptospira Serovars Canicola and Copenhageni from cattle urine in the state of Paraná, Brazil

Brazilian Journal of Microbiology

Francielle Gibson Da Silva Zacarias²  Silvio Arruda Vasconcellos¹  Eleine Kuroki Anzai²  Nilson Giraldi¹  Julio Cesar De Freitas²  Rudy Hartskeerl¹ 

[1] ,Universidade Estadual de Londrina Laboratório de Leptospirose Animal Programa de Pós-graduação em Ciência AnimalLondrina PR ,Brasil

关键词: Leptospira;    culture;    leptospirosis;    diagnosis;    cattle;    Leptospira;    cultura;    leptospirose;    diagnóstico;    bovino;   

DOI  :  10.1590/S1517-83822008000400028

来源: SciELO

【 摘 要 】

In 2001, 698 urine samples were randomly collected from cattle at a slaughterhouse in the State of Paraná, Brazil. Direct examination using dark field microscopy was carried out immediately after collection. Five putative positive samples were cultured in modified EMJH medium, yielding two positive cultures (LO-14 and LO-10). Typing with monoclonal antibodies revealed that the two isolates were similar to Canicola (LO-14) and Copenhageni (LO-10). Microscopic agglutination test results show that Hardjo is the most common serovar in cattle in Brazil. Rats and dogs are the common maintenance hosts of serovars Copenhageni and Canicola. The excretion of highly pathogenic serovars such as Copenhageni and Canicola by cattle can represent an increasing risk for severe leptospirosis is large populations, mainly living in rural areas.

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