The inflammasome adaptor ASC contributes to multiple innate immune processes in the resolution of otitis media:

Innate Immunity

ArwaKurabi¹ 

关键词: IL-1β;    cytokine;    middle ear;    inflammation;    macrophage;    NTHi;   

DOI  :  10.1177/1753425914526074

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

This study was designed to understand the contribution of the inflammasome and IL-1β activation in otitis media (OM). We examined the middle ear (ME) response to non-typeable Haemophilus influenzae (NTHi) in wild type (WT) mice using gene microarrays and a murine model of acute OM. Expression of members of the NOD domain-like receptor family of inflammasome genes was significantly up-regulated early in NTHi infection of the ME, potentially activating specific downstream regulatory cascades that contribute to the proliferative inflammatory response observed during OM. Expression of the pro-forms of the inflammasome targets IL-1β and IL-18 were also up-regulated. To evaluate the role of inflammasome-mediated cytokine maturation, NTHi-induced OM was examined in Asc−/−-deficient mice and compared with that seen in WT mice. Mice lacking the Asc gene showed near absence of IL-1β maturation in the ME and a reduction in leukocyte recruitment and infiltration to the cavity, and their macrophages exhibited reduced ...

【 授权许可】

CC BY