Innate Immunity | |
Analysis of IL-6 and IL-1β release in cryopreserved pooled human whole blood stimulated with endotoxin: | |
QingHe1  | |
关键词: Pyrogens; lipopolysaccharide; endotoxin; pro-inflammatory cytokines; fever; | |
DOI : 10.1177/1753425918777596 | |
学科分类:生物科学(综合) | |
来源: Sage Journals | |
【 摘 要 】
To overcome the lack of availability of fresh human whole blood for pyrogen detection, we explored the feasibility of utilizing cryopreserved pooled human blood to detect the responses of the pro-inflammatory cytokines IL-6 and IL-1β to LPS. Whole blood was obtained from five donors and incubated with LPS. The quantities of pro-inflammatory cytokines were measured using ELISA, and the results were compared among the samples. After the blood was cryopreserved with Dimethyl sulfoxide (DMSO) (10% v/v) and stored for 4 mo at –196℃, the detection limits of the IL-6/IL-1β responses to LPS were 0.2/0.4 endotoxin units (EU)/ml, respectively, and IL-6/IL-1β release increased in response to LPS in a dose-dependent manner. When these experiments were performed in three separate laboratories, the within-laboratory reproducibility of the IL-6/IL-1β responses was 100%/86.7%, 93.3%/100%, and 86.7%/80%, and the inter-laboratory reproducibility was 92.9%/85.7%, 64.3%/63.6%, and 57.1%/66.7%, respectively. The sensitivity (...
【 授权许可】
CC BY
【 预 览 】
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RO201901222829083ZK.pdf | 291KB | download |