Inhibitory role of cholinergic system mediated via α7 nicotinic acetylcholine receptor in LPS-induced neuro-inflammation:

Innate Immunity

EthikaTyagi¹ 

关键词: α7 Nicotinic acetylcholine receptor;    α4β2 nicotinic acetylcholine receptor;    lipopolysaccharide;    nicotine;    oxotremorine;    pro-inflammatory cytokines;   

DOI  :  10.1177/1753425909104680

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

This study investigated the influence of the cholinergic system on neuro-inflammation using nicotinic and muscarinic receptor agonists and antagonists. Intracerebroventricular (ICV) injection of lipopolysaccharide (LPS, 50 µg) was used to induce neuro-inflammation in rats and estimations of pro-inflammatory cytokines, α7 nicotinic acetylcholine receptor (nAChR) mRNA expression were done in striatum, cerebral cortex, hippocampus and hypothalamus at 24 h after LPS injection. Nicotine (0.2, 0.4 and 0.8 mg/kg, i.p.) or oxotremorine (0.2, 0.4 and 0.8 mg/kg, i.p.) were administered 2 h prior to sacrifice. We found that only nicotine was able to block the proinflammatory cytokines induced by LPS whereas, oxotremorine was found ineffective. Methyllycaconitine (MLA; 1.25, 2.5 and 5 mg/kg, i.p.), an α7 nAChR antagonist or dihydro-β-erythroidine (DHβE; 1.25, 2.5 and 5 mg/kg, i.p.), an α4β2 nAChR antagonist, was given 20 min prior to nicotine in LPS-treated rats. Methyllycaconitine antagonized the anti-inflammatory e...

【 授权许可】

CC BY