Porphyromonas gingivalis-impaired innate immune response via NLRP3 proteolysis in endothelial cells:

Innate Immunity

OlivierHuck¹ 

关键词: Inflammasome;    Porphyromonas gingivalis;    endothelial;    inflammation;    atherosclerosis;    NLRP3;   

DOI  :  10.1177/1753425914523459

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Porphyromonas gingivalis (Pg) is involved in the link between periodontal diseases and atherosclerosis worsening. In periodontal cells, Pg modifies IL-1β expression via the NLRP3 inflammasome pathway activation. Our aim was to investigate NLRP3 inflammasome activation in endothelial cells (ECs) after Pg infection and Pg-LPS stimulation. In both situations, RT-PCR experiments demonstrated an increase of the NLRP3 mRNA level that can be potentiated by pre-treatment of ECs with 5 mM ATP. However, Western blotting analysis revealed that Pg infection induced a proteolysis of NLRP3 protein and a major decrease of the native protein. After ATP pre-treatment and/or Pg-LPS stimulation, this proteolysis was not observed, while NLRP3 protein levels were increased. Proteolysis of the NLRP3 protein was not observed with heat-killed Pg and inhibition of ECs protein synthesis with cycloheximide did not abolish the NLRP3 protein degradation induced by Pg infection in ATP pre-treated cells. Additionally, significant incre...

【 授权许可】

CC BY