Antimicrobial properties of Tanacetum balsamita L. (Asteraceae) introduced in Ukrainian Polissya

Ukrainian Journal of Ecology

I. V. Ivashchenko¹ 

[1] Zhytomyr National Agroecological University

关键词: Tanacetum balsamita L.;    Asteraceae;    introduction;    extract;    microorganisms;    antimicrobial activity.;   

DOI  :  10.15421/20176

学科分类：生物科学（综合）

来源: Alex Matsyura Publishing

【 摘 要 】

The paper studies antimicrobial activity of ethanol extract ofTanacetum balsamitaL. from the family Asteraceae, tribus Anthemideae, the plant being medicinally and pharmaceutically promising. Antimicrobial properties ofTanacetum balsamitawere studied on test-cultures, collected from the Ukrainian Collection of Microorganisms (UCM, Institute of microbiology and virology SAS Ukraine), the test-cultures being ofEscherichia coli(coliform bacillius) UCM B-906 (ATCC 25922);Staphilococcus aureus(golden staphylococcus) UCM B-904 (ATCC 25923);Pseudomonas aeruginosa(blue pus bacillus) UCM B-900 (ATCC 9027);Candida albicansUCM Y-1918 (ATCC 885-653). The above microorganisms are test strains used to determine antimicrobial properties of medicines. The antimicrobial effect of the investigated substances was studied by the method of successive dilutions which determines minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). The extract has demonstrated selective antimicrobial activity against gram-positive bacteria strains ofStaphylococcus aureus . In comparison with the solvent, MIC and MBC values showed an 8-times increase.T. balsamitaextract revealed no bacteriostatic, bacteriocidal/fugicidal effect against gram-negative bacteria stains ofEscherichia coli, P. aeruginosaand fungusC. albicans . The antimicrobial activity of the extract againstS. aureusmay be attributed to the presence of essential oil and phenolic compounds. The major compounds in the essential oil are epoxylynaloolacetate (60.13 %) and b-thujone (29.7 %). The following phenolic compounds have been determined with the high-performance liquid chromatography method: flavonoids – luteolin, apigenin, diosmetin, acacetin, apigenin-7-bioside, acacetin-7-bioside, apigenin-7-glycoside, luteolin-7-glycoside, diosmetin-7-bioside, diosmetin-7-glycoside, diosmetin-7-rhamnoside, and hydroxycinnamic acids – n-coumaric, caffeic, chlorogenic, isochlorogenic. The dominant compound is isochlorogenic acid (35 % of the total phenolic compounds detected). The experimental data obtained gives evidence that further research and application ofT. balsamitais promising for creation of new anti-staphylococcus drugs.

【 授权许可】

CC BY