The nitrate metabolism enzyme indicator role in the environment state changes conditions

Ukrainian Journal of Ecology

A.Y. Pakhomov¹  O.M. Vasilyuk¹ 

[1] Oles Honchar Dnipro National University

关键词: heavy metals;    mammals;    maximum allowable concentration;    anthropogenic stress;    aspartate aminotransferase;    enzymes;   

DOI  :  10.15421/2018_239

学科分类：生物科学（综合）

来源: Alex Matsyura Publishing

【 摘 要 】

The paper reflects analyzes ofCdimpact on the total activity (nM pyruvic acid/ml s) of aspartate aminotransferase (AST, EC 2.6.1.1) nitrogen metabolism inGlechoma hederaceaL. leaves subject (as model) which dominated in the research area (in natural floodplain oak withStellaria holosteaL.) in conditions ofCdpollution (as anthropogenic press) and digging activity by Mammalia (as biotic action, withTalpa europaeaL., European mole, as model),) and their combine action. TheCdwas introduced in the form of saltsCd(NO 3 ) 2 in the concentrations: 0.25, 1.25 and 2,5 g/m2, equivalent to the inclusion ofCdin 1,5 and 10 doses of MAC on experimental sites. When addingCd , the content of doses (5 mg/kg soil MAC ofCd ) was taken into account. It was founded the increasing of the AST activity on 26% (with adding theCdsalts at a dose of 1 МAС and digging activity byTalpa europaeaL) according to control (1 MACCd ), witch it proved the non-specific reaction on stress. WithCdconcentration 5 and 10 MAC we observed the repression of the enzymes activity according to controls (5 and 10 MACCd ) on 10% and 50% in accordance. The protective properties byT. europaeaL. hadn’t positive results. The transferase enzyme activity according to another control (the area, is without pollution ofCdand digging activity byT. europaeaL.) reflected the increasing AST enzyme activity from 166% tо 218% (in presence 1 and 5 MACCd ) and reduction around 46% (in presence 10 MACCd ). The digging activity byT. europaeaL. lowered the toxic metal effect and the normalisation of the nitrogen metabolism by increasing the activity of AST from 55% to 266%, from 318% to 291% (AST, 1 та 5 MACCd ). The digging activity by Mammalia did not contribute the metal toxic effect under theCd10 MAC. Thus, using the different representatives of zoocoenosis promotes improvement in the Steppe Dnieper at low concentrations of the factor has been revealed.

【 授权许可】

CC BY