Effect of mammals’ digging function on alanine aminotransferase enzyme activity in leaves of Glechoma hederacea under cadmium pollution

Ukrainian Journal of Ecology

关键词: heavy metal;    mammals;    maximum allowable concentration;    anthropogenic stress;    aspartate aminotransferase;    enzymes;   

DOI  :  10.15421/2017_73

学科分类：生物科学（综合）

来源: Alex Matsyura Publishing

【 摘 要 】

The paper reflects analyzes ofCdimpact on the total activity (nM pyruvic acid/ml s) of Alanine aminotransferase (ALT, EC 2.6.1.2) nitrogen metabolism inGlechoma hederaceaL. leaves subject (as model) which dominated in the research area (in natural floodplain oak withStellaria holosteaL.) in conditions ofCdpollution (as anthropogenic press) and digging activity by Mammalia (as biotic action, withTalpa europaeaL., European mole, as model), and their combine action. TheCdwas introduced in the form of saltsCd(NO 3 ) 2in the concentrations:0.25, 1.25, and 2,5 g/m2, equivalent to the inclusion ofCdin 1,5 and 10 doses of MAC. The content of doses of MAC ofCd(5 mg/kg soil) adding took into account.It was found the increasing of the ALT activity on 88% (with adding theCdsalts at a dose of 1 МAС) and digging activity byTalpa europaeaL. which proved the non-specific reaction on stress. We observed the repression of the enzymes according to controls (5 and 10 MACCd ) withCdconcentration 5 and 10 MAC. The protective properties byT. europaeaL. hadn’t positive results. The transferase enzyme activity according to another control (area without pollution ofCdand digging activity byT. europaeaL.) reflected the inhibition of ALT on 78% tо 53% (in presenceCd1 and 5 MAC). The digging activity byT. europaeaL. promoted the toxic metal level and the normalisation of the nitrate metabolism from 25% tо 47% (ALT, 1 MACCd ). The digging activity by Mammalia did not contribute the metal toxic effect and restoration of the natural functions of the plant organism under theCd10 MAC. The advisability for using the representatives of zoocenosis for the complex regulation of environmental changes in the conditions of the Ukrainian Steppe, if the antropogenic factor does not exceeds the maximum permissible significance have been founded .

【 授权许可】

CC BY