Combined allelic dosage of Nfia and Nfib regulates cortical development:

Brain and Neuroscience Advances

JensBunt¹ 

关键词: Nuclear factor I;    nuclear factor I A;    nuclear factor I B;    neocortex;    hippocampus;    cortical development;    corpus callosum;   

DOI  :  10.1177/2398212817739433

学科分类：医学（综合）

来源: Sage Journals

【 摘 要 】

Background:Nuclear factor I family members nuclear factor I A and nuclear factor I B play important roles during cerebral cortical development. Nuclear factor I A and nuclear factor I B regulate similar biological processes, as their expression patterns, regulation of target genes and individual knockout phenotypes overlap. We hypothesised that the combined allelic loss of Nfia and Nfib would culminate in more severe defects in the cerebral cortex than loss of a single member.Methods:We combined immunofluorescence, co-immunoprecipitation, gene expression analysis and immunohistochemistry on knockout mouse models to investigate whether nuclear factor I A and nuclear factor I B function similarly and whether increasing allelic loss of Nfia and Nfib caused a more severe phenotype.Results:We determined that the biological functions of nuclear factor I A and nuclear factor I B overlap during early cortical development. These proteins are co-expressed and can form heterodimers in vivo. Differentially regulated ...

【 授权许可】

CC BY