Blood schizontocidal activity of methylene blue in combination with antimalarials against Plasmodium falciparum

Parasite

and E. Deharo²  A. Valentin²  I. Landau¹  M.C. Monje²  S. Maurel²  J. Rincon³  S. Bertani²  G. Garavito² 

[1] Laboratoire de Parasitologie Comparée et Modèles Expérimentaux, Muséum National d’Histoire Naturelle, USM 0307, 61, rue Buffon,75231 Paris Cedex 05,France;Pharmacochimie des Substances Naturelles et Pharmacophores Redox, Faculté des Sciences Pharmaceutiques, UMR-152 IRD-Université de Toulouse 3,31062 Toulouse Cedex 9,France;Departamento de Farmacia, Facultad de Ciencias, Universitad Nacional de Colombia,Carrera 30 45-03Bogotá D.C., Colombia

关键词: drug interactions;    methylene blue;    Plasmodium;    antimalarials;   

Others  :  808636
DOI  :  doi:10.1051/parasite/2007142135

 received in 2006-09-13, accepted in 2006-12-21,  发布年份 2007

【 摘 要 】

Methylene blue (MB) is the oldest synthetic antimalarial. It is not used anymore as antimalarial but should be reconsidered. For this purpose we have measured its impact on both chloroquine sensitive and resistant Plasmodium strains. We showed that around 5 nM of MB were able to inhibit 50% of the parasite growth in vitro and that late rings and early trophozoites were the most sensitive stages; while early rings, late trophozoites and schizonts were less sensitive. Drug interaction study following fractional inhibitory concentrations (FIC) method showed antagonism with amodiaquine, atovaquone, doxycycline, pyrimethamine; additivity with artemether, chloroquine, mefloquine, primaquine and synergy with quinine. These results confirmed the interest of MB that could be integrated in a new low cost antimalarial combination therapy.

【 授权许可】

   
© PRINCEPS Editions, Paris, 2007