Infective larvae of five Onchocerca species from experimentally infected Simulium species in an area of zoonotic onchocerciasis in Japan

Parasite

and O. Bain³  S. Uni¹  H. Takaoka²  M. Fukuda² 

[1] Department of Medical Zoology, Osaka City University Medical School,Osaka 545-8585,Japan;Department of Infectious Disease Control, Faculty of Medicine, Oita University,Hasama,Oita 879-5593,Japan;USM 307 Parasitologie comparée et Modèles expérimentaux, Muséum National d’Histoire Naturelle, 61, rue Buffon,75231 Paris Cedex 05,France

关键词: Japan;    zoonosis;    vector;    Simulium;    infective larvae;    experimental infection;    Onchocerca;   

Others  :  808536
DOI  :  doi:10.1051/parasite/2008152111

 received in 2007-10-15, accepted in 2007-12-21,  发布年份 2008

【 摘 要 】

Microfilariae of five Onchocerca species, O. dewittei japonica (the causative agent of zoonotic onchocerciasis in Oita, Kyushu, Japan) from wild boar (Sus scrofa), O. skrjabini and O. eberhardi from sika deer (Cervus nippon), O. lienalis from cattle, and an as yet unnamed Onchocerca sp. from wild boar, were injected intrathoracically into newly-emerged black flies of several species from Oita to search the potential vector(s) of these parasites and identify their infective larvae. Development of O. dewittei japonica microfilariae to the infective larvae occurred in Simulium aokii, S. arakawae, S. bidentatum, S. japonicum, S. quinquestriatum, and S. rufibasis while development of infective larvae of O. skrjabini, O. eberhardi, and the unnamed Onchocerca sp. was observed in S. aokii, S. arakawae, and S. bidentatum. Development of O. lienalis microfilaria to infective larvae occurred in S. arakawae. Based on the morphology of infective larvae obtained, we proposed a key of identification of Onchocerca infective larvae found in Oita. We also reconsider the identification of three types of infective larvae previously recovered from Simulium species captured at cattle sheds: the large type I larvae that may be an undescribed species; the small type III identified as O. lienalis may include O. skrjabini too; the intermediary type II that may be O. gutturosa, or O. dewittei japonica, or the unnamed Onchocerca sp. of wild boar.

【 授权许可】

   
© PRINCEPS Editions, Paris, 2008