Genetic evidence for the presence of two species of Onchocerca from the wild boar in Japan

Parasite

and H. Takaoka³  O. Bain¹  S. Uni²  Y. Otsuka³  M. Fukuda³ 

[1] Origine, Structure et Evolution de la biodiversité UMR 7205 CNRS et Muséum National d’Histoire Naturelle, Parasitologie Comparée,CP 52, 61, rue Buffon,75231 Paris Cedex 05,France;Department of Medical Zoology, Osaka City University Medical School,Osaka 545-8585,Japan;Department of Infectious Disease Control, Faculty of Medicine, Oita University, Hasama, Yufu,Oita 879-5593,Japan

关键词: genetic differences;    zoonosis;    vector;    Simulium;    infective larvae;    wild boar;    Onchocerca;   

Others  :  808353
DOI  :  doi:10.1051/parasite/2010171033

 received in 2009-09-09, accepted in 2009-11-02,  发布年份 2010

【 摘 要 】

In order to clarify the genetic differences between Onchocerca dewittei japonica, the causative agent of zoonotic onchocerciasis in Japan and a related undescribed Onchocerca sp., both parasitizing wild boar (Sus scrofa) of which the infective larval stages are indistinguishable from each other, we compared the sequences of the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene region from four infective larvae (recovered from experimentally infected black flies), one microfilaria, and one adult of O. dewittei japonica, and from one infective larva (recovered from an experimentally infected black fly), one microfilaria, and a pool of several microfilariae of O. sp. The length of the CO1 gene region was 649 bp for all samples but there was a difference of 8.8 to 9.4% in the sequences between the two species although there were intraspecific variations of 0 to 0.5%. The CO1 sequences of O. sp. did not correspond to any of those deposited in the databases. Our study provides evidence that O. dewittei japonica and O. sp. are genetically different from each other.

【 授权许可】

   
© PRINCEPS Editions, Paris, 2010