Prevalence of antibodies to Sarcocystis neurona and Neospora hughesi in horses from Mexico

Parasite

and Daniel K. Howe²  Jitender P. Dubey¹  Cosme Alvarado-Esquivel³  Michelle R. Yeargan² 

[1] United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001,Beltsville,Maryland 20705-2350,USA;Department of Veterinary Science, M.H. Gluck Equine Research Center, University of Kentucky,Lexington,Kentucky 40546-0099,USA;Faculty of Medicine and Nutrition, Biomedical Research Laboratory, Juárez University of Durango State,Avenida Universidad S/N,34000 Durango,Mexico

关键词: Surface antigens;    Central America;    ELISA;    Equine protozoal myeloencephalitis;    Seroprevalence;   

Others  :  808531
DOI  :  doi:10.1051/parasite/2013029

 received in 2013-06-17, accepted in 2013-08-27,  发布年份 2013

【 摘 要 】

Equine protozoal myeloencephalitis (EPM) is a debilitating disease of horses caused by Sarcocystis neurona and Neospora hughesi. Sera from 495 horses in Durango State, Mexico were tested for anti-protozoal antibodies using enzyme-linked immunosorbent assays (ELISAs) based on major surface antigens of these two parasites. Antibodies to S. neurona were detected in 240 (48.5%) of the 495 horse sera tested with the rSnSAG2/4/3 trivalent ELISA. Multivariate analysis showed that exposure to S. neurona was associated with age, feeding grains and crops, and small herd size. Antibodies to N. hughesi were found in 15 (3.0%) of the 495 horse sera tested with the rNhSAG1 ELISA and confirmed by Western blot of N. hughesi tachyzoite antigen. This is the first report of S. neurona and N. hughesi exposure in horses in Mexico, and it affirms that EPM should be in the differential diagnosis for horses exhibiting signs of neurologic disease in this country.

【 授权许可】

   
© M.R. Yeargan et al., published by EDP Sciences, 2013

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