Evaluation of a simple Theileria annulata culture protocol from experimentally infected bovine whole blood

Parasite

M.A. Darghouth¹  L. Sassi¹  R. Latrach¹  M. Gharbi¹ 

[1] Laboratoire de Parasitologie, Université de la Manouba, École Nationale de Médecine Vétérinaire,2020 Sidi Thabet,Tunisie

关键词: Ficoll;    blood;    cell culture;    Theileria annulata;   

Others  :  808800
DOI  :  doi:10.1051/parasite/2012193281

 received in 2011-12-19, accepted in 2012-02-23,  发布年份 2012

【 摘 要 】

We have evaluated a new simple technique using whole blood from experimentally infected cattle for the isolation and cultivation of Theileria annulata. The study was carried out on 20 Holstein-Frisian bovines that had been experimentally infected with a virulent lethal dose of Theileria annulata. This technique has been compared to the classical peripheral blood monocyte isolation with Ficoll carried out on 22 experimentally infected Holstein-Friesian calves. The effectiveness of the reference technique was estimated to 86.4%, whilst the effectiveness of the new technique was 100%. Moreover, this new technique leads to time and money saving estimated to € 3.06 per sample. It decreases the contamination risks by reducing the steps of sample manipulation.

【 授权许可】

   
© PRINCEPS Editions, Paris, 2012

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