A high frequency of MDSCs in sepsis patients, with the granulocytic subtype dominating in gram‐positive cases

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: MDSC;    cancer;    granulocyte;    CD14;    CD11c;    Ficoll;   

DOI  :  10.1189/jlb.5HI0214-074R

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

ThecausativemicroorganismsdictatethetypeofMDSCgeneratedinsepsispatients,andalargeproportionofPMN‐MDSCsingram‐positivesepsisincludesimmunosuppressivemyeloidblasts.MDSCsconstituteaheterogeneouspopulationofimmaturemyeloidcellsthatpotentlysuppressimmuneresponses.Theywereidentifiedoriginallyincancerpatientsandhavesincebeenreportedtooccuralsoinchronicinflammation,autoimmunity,andevenbacterialinfections.HumanMDSCsarecommonlydividedintoMo‐MDSCsandgranulocytic(PMN‐MDSCs)subtypes.TowhatextentthebonafidecancerMDSCsarerepresentativeoftheproposedMDSCsfoundinotherdiseasesisnotwellknown.PMN‐MDSCshavebeenfoundpreviouslytobeenrichedamongLDGsindensitygradient‐centrifugedblood.Inthisstudy,weanalyzedpotentialMDSCsinsepsispatientswithdifferentcausativemicroorganisms,usingtotalperipheralbloodcomparedwithdensitygradient‐centrifugedblood.WefoundahighfrequencyoftypicalCD14+HLA‐DRlowMo‐MDSCsinallsepsispatients,whereasthetypicalPMN‐MDSCs,aswellasaprominentCD14lowPMN‐MDSC‐likepopulation,appearedpreferentiallyingram‐positivecases.TheCD14lowPMN‐MDSCvariantwasdemonstratedtosuppressTcellproliferationinvitroviaaROS‐dependentmechanism,todisplayanincreasedIL‐10:TNF‐αratio,andtopresentwithsignsofimmaturity:blastmorphologyandlowcytokinelevels.WeconcludethataspectrumofcellswithMDSCfeaturesisenrichedinsepsisandthatthemicrobialoriginofsepsiscontributestothesubstantialinterindividualpatientvariationintheMDSCpattern...

【 授权许可】

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