Expression of EhRAD54, EhRAD51, and EhBLM proteins during DNA repair by homologous recombination in Entamoeba histolytica

Parasite

and Laurence A. Marchat³  Absalom Zamorano-Carrillo³  Cesar Lopez-Camarillo²  Marisol Pezet-Valdez¹  Lorena Garcia-Morales¹  Ma. del Socorro Charcas-Lopez¹ 

[1] Programa Institucional de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía del IPN, Guillermo Massieu Helguera No. 239, Fracc. La Escalera, Ticoman, México D.F., C.P. 07320,Mexico;Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, San Lorenzo No. 290, Col. del Valle, México D.F., C.P. 03110,Mexico;Programa de Doctorado en Biotecnología, Escuela Nacional de Medicina y Homeopatía del IPN, Guillermo Massieu Helguera No. 239, Fracc. La Escalera, Ticoman, México D.F., C.P. 07320,Mexico

关键词: Amoebiasis;    Homologous recombination;    DNA double-strand break repair;   

Others  :  808386
DOI  :  doi:10.1051/parasite/2014006

 received in 2013-10-23, accepted in 2014-02-07,  发布年份 2014

【 摘 要 】

Entamoeba histolytica, the protozoan responsible for human amoebiasis, exhibits a great genome plasticity that is probably related to homologous recombination events. It contains the RAD52 epistasis group genes, including Ehrad51 and Ehrad54, and the Ehblm gene, which are key homologous recombination factors in other organisms. Ehrad51 and Ehrad54 genes are differentially transcribed in trophozoites when DNA double-strand breaks are induced by ultraviolet-C irradiation. Moreover, the EhRAD51 recombinase is overexpressed at 30 min in the nucleus. Here, we extend our analysis of the homologous recombination mechanism in E. histolytica by studying EhRAD51, EhRAD54, and EhBLM expression in response to DNA damage. Bioinformatic analyses show that EhRAD54 has the molecular features of homologous proteins, indicating that it may have similar functions. Western blot assays evidence the differential expression of EhRAD51, EhRAD54, and EhBLM at different times after DNA damage, suggesting their potential roles in the different steps of homologous recombination in this protozoan.

【 授权许可】

   
© Ma. del Socorro Charcas-Lopez et al., published by EDP Sciences, 2014

【 图 表 】

Figure 1.

Figure 2.

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