Identification of EBV infection in adults with egg specific food allergy

Virology Journal

Lunan Wang²  Jinming Li²  Kuo Zhang²  Yuan Zhang¹  Zhiyang Nie³  Yang Pan¹ 

[1] Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People’s Republic of China;National Center for Clinical Laboratories, Beijing Hospital of the Ministry of Health, NO.1 Dahua Road, Beijing, People’s Republic of China;Department of Transfusion, Beijing Hospital of the Ministry of Health, Beijing, People’s Republic of China

关键词: Specific IgE;    Microrna;    Food allergy;    Epstein-Barr virus;   

Others  :  1152455
DOI  :  10.1186/1743-422X-10-9

 received in 2012-03-18, accepted in 2013-01-02,  发布年份 2013

【 摘 要 】

Background

Food allergy has been reported increasingly around the world during the past several decades. Epstein-Barr virus (EBV), a common herpesvirus with high infection rate, is now suspected to be a risk or protective factor in food allergy. The aim of the study was to investigate the possible role of EBV infection in IgE-mediated food allergy.

Methods

34 patients with an egg allergy and 34 healthy controls participated in this study. Egg allergy was confirmed by open-food challenge. Serum anti-viral capsid antigen (VCA), anti-Epstein-Barr nuclear antigen 1 (EBNA-1) IgG and egg specific (yolk and white)-IgE levels were evaluated by enzyme linked immunosorbent assay (ELISA). At the same time, EBV DNA as well as viral miRNAs in these samples was quantified by real-time PCR.

Results

The results showed that serum anti EBNA-1 IgG and two viral miRNAs (miR-BART1-5p and miR-BART7) were highly expressed in patients with egg allergy compared with healthy controls (p < 0.05, < 0.001 and < 0.01, respectively). Moreover, the expressions of anti EBNA-1 specific IgG, miR-BART1-5p and miR-BART7 positively correlated with the level of egg-specific IgE (p < 0.05, < 0.01 and < 0.01, respectively). The differences in anti VCA IgG concentration and EBV DNA copy number between the allergy patients and control individuals were not statistically significant.

Conclusions

The high expression of EBV-specific antibody and miRNAs indicated that EBV infection might play a promoting role in IgE-mediated egg food allergy, and viral miRNAs-related immunomodulatory pathway was likely involved in this allergy process.

【 授权许可】

   
2013 Pan et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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