Epilysin (matrix metalloproteinase-28) contributes to airway epithelial cell survival

Respiratory Research

William C Parks¹  Ann J Chen¹  Laura K Johnston¹  Susanna Harju-Baker¹  Anne M Manicone¹ 

[1] Center for Lung Biology, Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Washington, Seattle, WA, USA

关键词: Epithelium;    Apoptosis;    Influenza;    Matrix Metalloproteinases;   

Others  :  796778
DOI  :  10.1186/1465-9921-12-144

 received in 2011-07-15, accepted in 2011-10-31,  发布年份 2011

【 摘 要 】

MMP28 is constitutively expressed by epithelial cells in many tissues, including the respiratory epithelium in the lung and keratinocytes in the skin. This constitutive expression suggests that MMP28 may serve a role in epithelial cell homeostasis. In an effort to determine its function in epithelial cell biology, we generated cell lines expressing wild-type or catalytically-inactive mutant MMP28 in two pulmonary epithelial cell lines, A549 and BEAS-2B. We observed that over-expression of MMP28 provided protection against apoptosis induced by either serum-deprivation or treatment with a protein kinase inhibitor, staurosporine. Furthermore, we observed increased caspase-3/7 activity in influenza-infected lungs from Mmp28^-/- mice compared to wild-type mice, and this activity localized to the airway epithelium but was not associated with a change in viral load. Thus, we have identified a novel role of MMP28 in promoting epithelial cell survival in the lung.

【 授权许可】

   
2011 Manicone et al; licensee BioMed Central Ltd.

【 图 表 】

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