Altered gene and protein expression in liver of the obese spontaneously hypertensive/NDmcr-cp rat

Nutrition & Metabolism

Sahoko Ichihara⁴  Masao Yuda⁸  Ichiro Takeuchi⁷  Emiko Kitagawa¹  Hitoshi Iwahashi⁶  Saeko Tada-Oikawa²  Yoshiji Yamada⁴  Yasuhiro Hotta⁴  Yui Nanpei⁴  Gaku Ichihara³  Shinji Oikawa⁵  Jie Chang³ 

[1] Applied Science, Roche Diagnostics, Tokyo, Japan;Graduate School of Regional Innovation Studies, Mie University, 1577 Kurimamachiya-cho, Tsu 514-8507, Japan;Department of Environmental and Occupational Health, Nagoya University Graduate School of Medicine, Nagoya, Japan;Department of Human Functional Genomics, Life Science Research Center, Mie University, Tsu, Japan;Department of Molecular and Environmental Medicine, Mie University Graduate School of Medicine, Tsu, Japan;Graduate School of Applied Biological Sciences, Gifu University, Gifu, Japan;Department of Engineering, Nagoya Institute of Technology, Nagoya, Japan;Department of Medical Zoology, Mie University Graduate School of Medicine, Tsu, Japan

关键词: Liver;    Obesity;    Proteomics analysis;    Microarray analysis;    Pathophysiology;    Metabolic syndrome;   

Others  :  811974
DOI  :  10.1186/1743-7075-9-87

 received in 2012-04-24, accepted in 2012-09-07,  发布年份 2012

【 摘 要 】

Background

It is difficult to study the mechanisms of the metabolic syndrome in humans due to the heterogeneous genetic background and lifestyle. The present study investigated changes in the gene and protein profiles in an animal model of the metabolic syndrome to identify the molecular targets associated with the pathogenesis and progression of obesity related to the metabolic syndrome.

Methods

We extracted mRNAs and proteins from the liver tissues of 6- and 25-week-old spontaneously hypertensive/NIH –corpulent rat SHR/NDmcr-cp (CP), SHR/Lean (Lean) and Wistar Kyoto rats (WKY) and performed microarray analysis and two-dimensional difference in gel electrophoresis (2D-DIGE) linked to a matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS).

Results

The microarray analysis identified 25 significantly up-regulated genes (P < 0.01; log₁₀ > 1) and 31 significantly down-regulated genes (P < 0.01; log₁₀ < −1) in 6- and 25-week-old CP compared with WKY and Lean. Several of these genes are known to be involved in important biological processes such as electron transporter activity, electron transport, lipid metabolism, ion transport, transferase, and ion channel activity. MALDI-TOF/TOF MS identified 31 proteins with ±1.2 fold change (P < 0.05) in 6- and 25-week-old CP, compared with age-matched WKY and Lean. The up-regulated proteins are involved in metabolic processes, biological regulation, catalytic activity, and binding, while the down-regulated proteins are involved in endoplasmic reticulum stress-related unfolded protein response.

Conclusion

Genes with significant changes in their expression in transcriptomic analysis matched very few of the proteins identified in proteomics analysis. However, annotated functional classifications might provide an important reference resource to understand the pathogenesis of obesity associated with the metabolic syndrome.

【 授权许可】

   
2012 Chang et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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