Wolbachia detection in insects through LAMP: loop mediated isothermal amplification

Particle and Fibre Toxicology

Luciano Andrade Moreira²  Nilton Barnabé Rodrigues¹  Caroline Dantas de Oliveira²  Anna Paula Alvim Cassimiro²  Daniela da Silva Gonçalves² 

[1] Entomologia Médica, Centro de Pesquisas René Rachou - Fundação Oswaldo Cruz, Av. Augusto de Lima 1715, 30190-002 Belo Horizonte, MG, Brazil;Grupo: Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor, Centro de Pesquisas René Rachou - Fundação Oswaldo Cruz, Av. Augusto de Lima 1715, 30190-002 Belo Horizonte, MG, Brazil

关键词: Insect;    Mosquito;    LAMP-loop mediated isothermal amplification;    Wolbachia;   

Others  :  806649
DOI  :  10.1186/1756-3305-7-228

 received in 2014-03-25, accepted in 2014-05-06,  发布年份 2014

【 摘 要 】

Background

The bacterium Wolbachia is a promising agent for the biological control of vector-borne diseases as some strains have the ability to block the transmission of key human disease-causing pathogens. Fast, accurate and inexpensive methods of differentiating between infected and uninfected insects will be of critical importance as field-based trials of Wolbachia-based bio-control become increasingly common.

Findings

We have developed a specific and sensitive method of detecting Wolbachia based on the isothermal DNA amplification. This technique can be performed in an ordinary heat block without the need for gel-based visualisation, and is effective for a wide variety of insect hosts.

Conclusion

Here we present the development of a rapid, highly sensitive and inexpensive method to detect Wolbachia in a variety of insect hosts, including key mosquito disease vectors.

【 授权许可】

   
2014 Gonçalves et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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【 参考文献 】

• [1]McGraw EA, O’Neill SL: Beyond insecticides: new thinking on an ancient problem. Nat Rev Microbiol 2013, 11:181-193.
• [2]Kambris Z, Cook PE, Phuc HK, Sinkins SP: Immune activation by life-shortening Wolbachia and reduced filarial competence in mosquitoes. Science 2009, 326(80):134-136.
• [3]Bian G, Xu Y, Lu P, Xie Y, Xi Z: The Endosymbiotic Bacterium Wolbachia induces resistance to Dengue Virus in Aedes aegypti. PLoS Pathog 2010, 6:e1000833.
• [4]Glaser RL, Meola MAC-P: The native Wolbachia endosymbionts of Drosophila melanogaster and Culex quinquefasciatus increase host resistance to West Nile virus infection. PLoS One 2010, 5:e11977.
• [5]Bian G, Joshi D, Dong Y, Lu P, Zhou G, Pan X, Xu Y, Dimopoulos G, Xi Z: Wolbachia invades Anopheles stephensi populations and induces refractoriness to Plasmodium infection. Science 2013, 340(80-):748-751.
• [6]Werren JH, Baldo L, Clark ME: Wolbachia: master manipulators of invertebrate biology. Nat Rev Microbiol 2008, 6:741-751.
• [7]Hertig M, Wolbach SB: Studies on Rickettsia-like micro-organisms in insects *. J Med Res 1924, 44:329-374.7.
• [8]Zug R, Hammerstein P: Still a host of hosts for Wolbachia: analysis of recent data suggests that 40% of terrestrial arthropod species are infected. PLoS One 2012, 7:e38544.
• [9]Kittayapong P, Baisley KJ, Baimai V, O’Neill SL: Distribution and diversity of Wolbachia infections in Southeast Asian mosquitoes (Diptera: Culicidae). J Med Entomol 2000, 37:340-345.
• [10]Zhou W, Rousset F, O’Neil S: Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences. Proc Biol Sci 1998, 265:509-515.
• [11]Werren JH, Zhang W, Guo LR: Evolution and phylogeny of Wolbachia: reproductive parasites of arthropods. Proc Biol Sci 1995, 261:55-63.
• [12]Simoes P, Mialdea G, Reiss D, Sagot F, Charlat S: Wolbachia detection: an assessment of standard PCR Protocols. Mol Ecol Resour 2011, 11:567-572.
• [13]Casper-Lindley C, Kimura S, Saxton DS, Essaw Y, Simpson I, Tan V, Sullivan W: Rapid fluorescence-based screening for Wolbachia endosymbionts in Drosophila germ line and somatic tissues. Appl Environ Microbiol 2011, 77:4788-4794.
• [14]Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T: Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 2000, 28:e63.
• [15]Fu S, Qu G, Guo S, Ma L, Zhang N, Zhang S, Gao SSZ: Applications of loop-mediated isothermal DNA amplification. Appl Biochem Biotechnol 2011, 163:845-850.
• [16]Goto M, Honda E, Ogura A, Nomoto A, Hanaki K-I: Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue. Biotechniques 2009, 46:167-172.
• [17]Moreira LA, Iturbe-Ormaetxe I, Jeffery JA, Lu G, Pyke AT, Hedges LM, Rocha BC, Hall-Mendelin S, Day A, Riegler M, Hugo LE, Johnson KN, Kay BH, McGraw EA, Van den Hurk AF, Ryan PA, O’Neill SL: A Wolbachia symbiont in Aedes aegypti limits infection with Dengue, Chikungunya, and Plasmodium. Cell 2009, 139:1268-1278.
• [18]McMeniman CJ, Lane AM, Fong AW, Voronin DA, Iturbe-Ormaetxe I, Yamada R, McGraw EA, O’Neill SL: Host adaptation of a Wolbachia strain after long-term serial passage in mosquito cell lines. Appl Env Microbiol 2008, 74:6963-6969.
• [19]Walker T, Moreira LA: Can Wolbachia be used to control malaria? Mem Inst Oswaldo Cruz 2011, 106:212-217.
• [20]Baton LA, Pacidônio EC, Gonçalves DDS, Moreira LA: wFlu: characterization and evaluation of a native Wolbachia from the mosquito Aedes fluviatilis as a potential vector control agent. PLoS One 2013, 8:e59619.
• [21]Fu Y, Gavotte L, Mercer DR, Dobson SL: Artificial triple Wolbachia infection in Aedes albopictus yields a new pattern of unidirectional cytoplasmic incompatibility. Appl Environ Microbiol 2010, 76:5887-5891.
• [22]Khan MGM, Bhaskar KRH, Salam MA, Akther T, Pluschke G, Mondal D: Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for detection of Leishmania DNA in bufft coat from visceral leishmaniasis patients. Parasites & Vectors 2012, 5:280. BioMed Central Full Text
• [23]Kong QM, Lu SH, Tong QB, Lou D, Chen R, Zheng B, Kumagai T, Wen LY, Ohta N, Zhou XN: Loop-mediated isothermal amplification (LAMP): early detection of Toxoplasma gondii infection in mice. Parasites & Vectors 2012, 5:2. BioMed Central Full Text
• [24]Song L, Li J, Hou S, Li X, Chen S: Establishment of loop-mediated isothermal amplification (LAMP) for rapid detection of Brucella spp. and application to milk and blood samples. J Microbiol Methods 2012, 90:292-297.
• [25]Ma M, Liu Z, Sun M, Yang J, Guan G, Li Y, Luo J, Yin H: Development and evaluation of a loop-mediated isothermal amplification method for rapid detection of Anaplasma ovis. J Clin Microbiol 2011, 49:2143-2146.
• [26]Nakao R, Stromdahl EY, Magona JW, Faburay B, Namangala B, Malele I, Inoue N, Geysen D, Kajino K, Jongejan F, Sugimoto C: Development of loop-mediated isothermal amplification (LAMP) assays for rapid detection of Ehrlichia ruminantium. BMC Microbiol 2010, 10:296. BioMed Central Full Text
• [27]Minard G, Mavingui P, Moro CV: Diversity and function of bacterial microbiota in the mosquito holobiont. Parasit Vectors 2013, 6:146. BioMed Central Full Text
• [28]Dunning Hotopp JC, Clark ME, Oliveira DCSG, Foster JM, Fischer P, Muñoz Torres MC, Giebel JD, Kumar N, Ishmael N, Wang S, Ingram J, Nene RV, Shepard J, Tomkins J, Richards S, Spiro DJ, Ghedin E, Slatko BE, Tettelin H, Werren JH: Widespread lateral gene transfer from intracellular bacteria to multicellular eukaryotes. Science 2007, 317:1753-1756.