Detection of border disease virus (BDV) in goat herds suffering diarrhea in eastern China

Virology Journal

Jieyuan Jiang²  Kongwang He²  Yinhua Sun¹  Yongqian Zhao²  Li Mao²  Wenliang Li² 

[1] Hai’an animal husbandry and veterinary station, Hai’an, 226600, China;Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences; Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture; National Center for Engineering Research of Veterinary Bio-products, Nanjing, 210014, China

关键词: Eastern China;    Phylogenetic analysis;    Diarrhea;    Goat;    Border disease virus;   

Others  :  1151336
DOI  :  10.1186/1743-422X-10-80

 received in 2012-10-17, accepted in 2013-03-04,  发布年份 2013

【 摘 要 】

Background

Border disease virus (BDV) is an important pathogen in sheep and goat production. Neither epidemiological investigation nor any reports of BDV infection was available in China. During Jan to Apr, 2012, several herd goats in Anhui and Jiangsu provinces in eastern China suffered unremitting diarrhea, with morbidity and mortality of about 28-37% and 10-15%, respectively. In the present study, sera and tissue samples from diseased goats of four farms were taken for BDV detection, isolation and identification.

Results

Panpesti generic primers and border disease virus (BDV)-specific primers targeting the 5’-UTR region produced RT-PCR positive bands for sera (24/28) and tissue samples (7/30). Twenty positive sera and tissue samples were inoculated onto Madin-Darby bovine kidney (MDBK) cells for virus isolation. Finally, three different strains of BDV, named AH12-01, AH12-02 and JS12/04, were successfully isolated as identified by RT-PCR using 5’-UTR and N^pro gene primers, sequencing and electron microscopy. Sequences of 5’-UTR and N^pro genes of them were used for phylogenetic analysis and comparison to other reference sequences available in GenBank. The results indicated AH12-01, AH12-02 and JS12/04 possess high relationship with the BDV 3 group viruses and differed with each other.

Conclusion

This is the first detection of BDV from goats with diarrhea and confirmation of BDV infection in China.

【 授权许可】

   
2013 Li et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

【 参考文献 】

• [1]Oguzoglu TC, Tan MT, Toplu N, Demir AB, Bilge-Dagalp S, Karaoglu T, Ozkul A, Alkan F, Burgu I, Haas L, Greiser-Wilke I: Border disease virus (BDV) infections of small ruminants in Turkey: a new BDV subgroup? Vet Microbiol 2009, 135:374-379.
• [2]Peterhans E, Schweizer M: Pestiviruses: how to outmaneuver your hosts. Vet Microbiol 2010, 142:18-25.
• [3]Becher P, Orlich M, Thiel HJ: Complete genomic sequence of border disease virus, a pestivirus from sheep. J Virol 1998, 72:5165-5173.
• [4]Barlow RM, Gardiner AC, Nettleton PF: The pathology of a spontaneous and experimental mucosal disease-like syndrome in sheep recovered from clinical border disease. J Comp Pathol 1983, 93:451-461.
• [5]Monies RJ, Paton DJ, Vilcek S: Mucosal disease-like lesions in sheep infected with Border disease virus. Vet Rec 2004, 155:765-769.
• [6]Cabezon O, Rosell R, Velarde R, Mentaberre G, Casas-Diaz E, Lavin S, Marco I: Border disease virus shedding and detection in naturally infected Pyrenean chamois (Rupicapra pyrenaica). J Vet Diagn Invest 2010, 22:744-747.
• [7]Giangaspero M, Ibata G, Savini G, Osawa T, Tatami S, Takagi E, Moriya H, Okura N, Kimura A, Harasawa R: Epidemiological survey of Border disease virus among sheep from northern districts of Japan. J Vet Med Sci 2011, 73:1629-1633.
• [8]Albayrak H, Gumusova SO, Ozan E, Yazici Z: Molecular detection of pestiviruses in aborted foetuses from provinces in northern Turkey. Trop Anim Health Prod 2012, 44:677-680.
• [9]McFadden A, Tisdall D, Hill F, Otterson P, Pulford D, Peake J, Finnegan C, La Rocca S, Kok-Mun T, Weir A: The first case of a bull persistently infected with Border disease virus in New Zealand. N Z Vet J 2012, 60:290-296.
• [10]Dubois E, Russo P, Prigent M, Thiery R: Genetic characterization of ovine pestiviruses isolated in France, between 1985 and 2006. Vet Microbiol 2008, 130:69-79.
• [11]Vilcek S, Nettleton PF, Paton DJ, Belak S: Molecular characterization of ovine pestiviruses. J Gen Virol 1997, 78:725-735.
• [12]Strong R, La Rocca SA, Ibata G, Sandvik T: Antigenic and genetic characterisation of border disease viruses isolated from UK cattle. Vet Microbiol 2010, 141:208-215.
• [13]Chalmers GA, Nation PN, Pritchard J: Border disease - a cause of terminal ileitis in lambs? Can Vet J 1990, 31:611.
• [14]Krametter-Froetscher R, Duenser M, Preyler B, Theiner A, Benetka V, Moestl K, Baumgartner W: Pestivirus infection in sheep and goats in West Austria. Vet J 2010, 186:342-346.
• [15]Toplu N, Oguzoglu TÇ, Epikmen ET, Aydogan A: Neuropathologic study of border disease virus in naturally infected fetal and neonatal small ruminants and its association with apoptosis. Vet Pathol 2011, 48:576-583.
• [16]Pratelli A, Martella V, Cirone F, Buonavoglia D, Elia G, Tempesta M, Buonavoglia C: Genomic characterization of pestiviruses isolated from lambs and kids in southern Italy. J Virol Methods 2001, 94:81-85.
• [17]Thabti F, Letellier C, Hammami S, Pepin M, Ribiere M, Mesplede A, Kerkhofs P, Russo P: Detection of a novel border disease virus subgroup in Tunisian sheep. Arch Virol 2005, 150:215-229.
• [18]Vilcek SHA, Herring JA, Nettleton PF, Lowings JP, Paton DJ: Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch Virol 1994, 136:309-323.
• [19]Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG: The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997, 25:4876-4882.
• [20]Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 2007, 24:1596-1599.