First study conducted in Northern India that identifies group C rotavirus as the etiological agent of severe diarrhea in children in Delhi

Virology Journal

Baoming Jiang¹  Pratima Ray²  Arvind Bagga³  Maharaj Kishen Bhan³  Vasundhara Razdan Tiku³  Satender Aneja⁴  Praveen Kumar⁴ 

[1] Centers for Disease Control and Prevention;Department of Biotechnology, Faculty of Science, Jamia Hamdard University;Department of Pediatrics, All India Institute of Medical Sciences;Kalawati Saran Children’s Hospital, Lady Hardinge Medical College;

关键词: Diarrhea;    Children;    Group C Rotavirus;    Gastroenteritis;    Phylogenetic analysis;    Sequence identity matrix;   

DOI  :  10.1186/s12985-017-0767-8

来源: DOAJ

【 摘 要 】

Abstract Background Group C Rotavirus (RVC) is an enteric pathogen responsible for acute gastroenteritis in children and adults globally. At present there are no surveillance studies on group C Rotaviruses in India and therefore their prevalence in India remains unknown. The present study aimed to evaluate group C rotavirus infection among <5 years old children hospitalized with acute gastroenteritis in New Delhi. Methods A total of 350 fecal specimens were collected during September 2013 to November 2014 from <5 years old diarrheal patients admitted at KSCH hospital, Delhi. The samples found negative for group A rotavirus (N = 180) by Enzyme immunoassay were screened for group C rotavirus by RT-PCR with VP6, VP7 and VP4 gene specific primers. The PCR products were further sequenced (VP6, VP7, VP4) and analyzed to ascertain their origin and G and P genotypes. Results Six out of 180 (group A rotavirus negative) samples were found positive for group C rotavirus by VP6 gene specific RT-PCR, of which 3 were also found positive for VP7 and VP4 genes. Phylogenetic analysis of VP7 and VP4 genes of these showed them to be G4 and P[2] genotypes. Overall, the nucleotide sequence data (VP6, VP7 and VP4) revealed a close relationship with the human group C rotavirus with no evidence of animal ancestry. Interestingly, the nucleotide sequence analysis of various genes also indicated differences in their origin. While the identity matrix of VP4 gene (n = 3) showed high amino acid sequence identity (97.60 to 98.20%) with Korean strain, the VP6 gene (n = 6) showed maximum identity with Nigerian strain (96.40 to 97.60%) and VP7 gene (n = 3) with Bangladeshi and USA strains. This is true for all analyzed samples. Conclusion Our study demonstrated the group C rotavirus as the cause of severe diarrhea in young children in Delhi and provides insights on the origin of group C rotavirus genes among the local strains indicating their source of transmission. Our study also highlights the need for a simple and reliable diagnostic test that can be utilized to determine the disease burden due to group C rotavirus in India.

【 授权许可】

Unknown