Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions

Proteome Science

Chin-Rang Yang³  David A Boothman⁴  Minzi Ruan⁵  Amyn A Habib²  Makoto Kuro-o¹  Johanne Pastor¹  Yonglong Zou⁴  Ameena J Jiwani²  Ying Dong⁴  Xiaoyu Wang³ 

[1] Department of Pathology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA;North Texas VA Healthcare System, Dallas, TX 75216, USA;Department of Radiation Oncology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA;Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA;VigeneTech, Inc. Carlisle, MA 01741, USA

关键词: systems biology;    glioma;    image analysis;    cross-talk;    signaling pathway;    quantitative analysis;    quantum dot;    protein array;   

Others  :  821026
DOI  :  10.1186/1477-5956-9-53

 received in 2011-04-21, accepted in 2011-09-15,  发布年份 2011

【 摘 要 】

An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity than traditional Western blots, but are not linear by the enzyme-based amplification. Software is developed to facilitate the quantitative readouts of signaling network activities. Kinetics of EGFRvIII mutant signaling was analyzed to quantify cross-talks between EGFR and other signaling pathways.

【 授权许可】

   
2011 Wang et al; licensee BioMed Central Ltd.

【 图 表 】

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