Further spreading of canine oriental eyeworm in Europe: first report of Thelazia callipaeda in Romania

Particle and Fibre Toxicology

Angela Monica Ionică³  Ioana Adriana Matei³  Ramona Chirilă¹  Iuliu Scurtu²  Gianluca D’Amico³  Andrei Daniel Mihalca³ 

[1] Faculty of Environmental Protection, University of Oradea, Strada General Magheru, Oradea 410048, Romania;Department of Internal Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Faculty of Veterinary Medicine, Calea Mănăștur 3-5, Cluj-Napoca 400372, Romania;Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Faculty of Veterinary Medicine, Calea Mănăștur 3-5, Cluj-Napoca 400372, Romania

关键词: Canine vector-borne diseases;    Emerging disease;    Thelazia callipaeda;   

Others  :  1147463
DOI  :  10.1186/s13071-015-0663-2

 received in 2014-11-13, accepted in 2015-01-15,  发布年份 2015

【 摘 要 】

Background

Despite the increasing number of reports of autochthonous cases of ocular thelaziosis in dogs in several European countries, and the evident emergence of human cases, the distribution and spreading potential of this parasite is far for being fully known. In Romania, despite intensive surveillance performed over recent years on the typical hosts of T. callipaeda, the parasite has not been found until now.

Methods

In October 2014 a German Shepherd was presented for consultation to a private veterinary practice from western Romania with a history of unilateral chronic conjunctivitis. Following a close examination of the affected eye, nematodes were noticed in the conjunctival sac. The specimens collected were used for morphological examination (light microscopy) and molecular analysis (amplification of the cytochrome c oxidase subunit 1 gene, followed by sequencing).

Results

Thirteen nematodes were collected, all identified morphologically as T. callipaeda. The history of the dog revealed no travel outside Romania, and during the last year, not even outside the home locality. The BLAST analysis of our sequence showed a 100% similarity T. callipaeda haplotype h1.

Conclusions

This is the first report of T. callipaeda in Romania, which we consider to be with autochthonous transmission. These findings confirm the spreading trend of T callipaeda and the increased risk of emerging vector-borne zoonoses.

【 授权许可】

   
2015 Mihalca et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Otranto D, Traversa D. Thelazia eyeworm: an original endo- and ecto-parasitic nematode. Trends Parasitol. 2005; 21:1-4.
• [2]Hodžić A, Latrofa MS, Annoscia G, Alić A, Beck R, Lia RP, Dantas-Torres F, Otranto D. The spread of zoonotic Thelazia callipaeda in the Balkan area. Parasit Vectors. 2014; 7:352. BioMed Central Full Text
• [3]Anderson RC. Nematode Parasites of Vertebrates: Their Development and Transmission. CABI Publishing, Guilford; 2000.
• [4]Rossi L, Bertaglia PP. Presence of Thelazia callipaeda Railliet & Henry, 1910, in Piedmont, Italy. Parassitologia. 1989; 31:167-172.
• [5]Di Sacco B, Ciocca A, Sirtori G. Thelazia callipaeda (Railliet and Henry, 1910) nel sacco congiuntivale di un gatto di Milano. Veterinaria. 1995; 4:81-84. in Italian, with abstract in English
• [6]Lia RP, Garaguso M, Otranto D, Puccini V. First report of Thelazia callipaeda in Southern Italy, Basilicata region. Acta Parasitol. 2000; 45:178.
• [7]Otranto D, Ferroglio E, Lia RP, Traversa D, Rossi L. Current status and epidemiological observation of Thelazia callipaeda (Spirurida, Thelaziidae) in dogs, cats and foxes in Italy: a “coincidence” or a parasitic disease of the Old Continent? Vet Parasitol. 2003; 116:315-325.
• [8]Otranto D, Lia RP, Cantacessi C, Testini G, Troccoli A, Shen JL, Wang ZX. Nematode biology and larval development of Thelazia callipaeda (Spirurida, Thelaziidae) in the drosophilid intermediate host in Europe and China. Parasitology. 2005; 131:847-855.
• [9]Otranto D, Cantacessi C, Testini G, Lia RP. Phortica variegata as an intermediate host of Thelazia callipaeda under natural conditions: evidence for pathogen transmission by a male arthropod vector. Int J Parasitol. 2006; 36:1167-1173.
• [10]Dorchies P, Chaudieu G, Siméon LA, Cazalot G, Cantacessi C, Otranto D. First reports of autochthonous eyeworm infection by Thelazia callipaeda (Spirurida, Thelaziidae) in dogs and cat from France. Vet Parasitol. 2007; 149:294-297.
• [11]Malacrida F, Hegglin D, Bacciarini L, Otranto D, Nägeli F, Nägeli C, Bernasconi C, Scheu U, Balli A, Marenco M, Togni L, Deplazes P, Schnyder M. Emergence of canine ocular thelaziosis caused by Thelazia callipaeda in southern Switzerland. Vet Parasitol. 2008; 157:321-327.
• [12]Magnis J, Naucke TJ, Mathis A, Deplazes P, Schnyder M. Local transmission of the eye worm Thelazia callipaeda in southern Germany. Parasitol Res. 2010; 106:715-717.
• [13]Miró G, Montoya A, Hernández L, Dado D, Vázquez MV, Benito M, Villagrasa M, Brianti E, Otranto D. Thelazia callipaeda: infection in dogs: a new parasite for Spain. Parasit Vectors. 2011; 4:148. BioMed Central Full Text
• [14]Vieira L, Rodrigues FT, Costa A, Diz-Lopes D, Machado J, Coutinho T, Tuna J, Latrofa MS, Cardoso L, Otranto D. First report of canine ocular thelaziosis by Thelazia callipaeda in Portugal. Parasit Vectors. 2012; 5:124. BioMed Central Full Text
• [15]Otranto D, Testini G, De Luca F, Hu M, Shamsi S, Gasser RB. Analysis of genetic variability within Thelazia callipaeda (Nematoda: Thelazioidea) from Europe and Asia by sequencing and mutation scanning of the mitochondrial cytochrome c oxidase subunit 1 gene. Mol Cell Probes. 2005; 19:306-313.
• [16]Kiss T, Cadar D, Krupaci AF, Bordeanu A, Brudaşcă GF, Mihalca AD, Mircean V, Gliga L, Dumitrache MO, Spînu M. Serological reactivity to Borrelia burgdorferi sensu lato in dogs and horses from distinct areas in Romania. Vector Borne Zoonotic Dis. 2011; 11:1259-1262.
• [17]Mircean V, Dumitrache MO, Györke A, Pantchev N, Jodies R, Mihalca AD, Cozma V. Seroprevalence and geographic distribution of Dirofilaria immitis and tick-borne infections (Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, and Ehrlichia canis) in dogs from Romania. Vector Borne Zoonotic Dis. 2012; 12:595-604.
• [18]Dumitrache MO, Kiss B, Dantas-Torres F, Latrofa MS, D’Amico G, Sándor AD, Mihalca AD. Seasonal dynamics of Rhipicephalus rossicus attacking domestic dogs from the steppic region of southeastern Romania. Parasit Vectors. 2014; 7:97. BioMed Central Full Text
• [19]Dumitrache MO, D’Amico G, Matei IA, Ionică A, Gherman CM, Sikó Barabási S, Ionescu DT, Oltean M, Balea A, Ilea IC, Sándor AD, Mihalca AD. Ixodid ticks in red foxes (Vulpes vulpes) from Romania. Parasit Vectors. 2014; 7 Suppl 1:1. BioMed Central Full Text
• [20]Mircean V, Dumitrache MO, Mircean M, Bolfa P, Györke A, Mihalca AD. Autochthonous canine leishmaniasis in Romania: neglected or (re)emerging? Parasit Vectors. 2014; 7:135. BioMed Central Full Text
• [21]Ionică AM, D’Amico G, Mitková B, Kalmár Z, Annoscia G, Otranto D, Modrý D, Mihalca AD. First report of Cercopithifilaria spp. in dogs from Eastern Europe with an overview of their geographic distribution in Europe. Parasitol Res. 2014; 113:2761-2764.
• [22]Ionică AM, Matei IA, Mircean V, Dumitrache MO, Annoscia G, Otranto D, Modrý D, Mihalca AD. Filarial infections in dogs from Romania: a broader view. In Proceedings of Fourth European Dirofilaria and Angiostrongylus Days: 2-4 July 2014; Budapest. 1996:42.
• [23]Sándor AD, Dumitrache MO, D’Amico G, Kiss BJ, Mihalca AD. Rhipicephalus rossicus and not R. sanguineus is the dominant tick species of dogs in the wetlands of the Danube Delta, Romania. Vet Parasitol. 2014; 204:430-432.
• [24]Mitková B, Qablan MA, Mihalca AD, Modrý D. Questing for the identity of Hepatozoon in foxes. Parasit Vectors. 2014; 7 Suppl 1:O23. BioMed Central Full Text
• [25]Casiraghi M, Anderson TJC, Bandi C, Bazzocchi C, Genchi C. A phylogenetic analysis of filarial nematodes: comparison with the phylogeny of Wolbachia endosymbionts. Parasitology. 2001; 122:93-103.
• [26]Otranto D, Lia RP, Traversa D, Giannetto S. Thelazia callipaeda (Spirurida, Thelaziidae) of carnivores and humans: morphological study by light and scanning electron microscopy. Parassitologia. 2003; 45:125-133.
• [27]TaxoDros: The database on Taxonomy of Drosophilidae. http://taxodros.uzh.ch. Accessed 12.01.2015.
• [28]Ruytoor P, Déan E, Pennant O, Dorchies P, Chermette R, Otranto D, Guillot J. Ocular thelaziosis in dogs, France. Emerg Infect Dis. 2010; 16:1943-1945.
• [29]Rodrigues FT, Cardoso L, Coutinho T, Otranto D, Diz-Lopes D. Ocular thelaziosis due to Thelazia callipaeda in a cat from northeastern Portugal. J Feline Med Surg. 2012; 14:952-954.
• [30]Soares C, Sousa SR, Anastacio S, Matias MG, Marques I, Mascarenhas S, Vieira MJ, de Carvalho LM, Otranto D. Feline thelaziosis caused by Thelazia callipaeda in Portugal. Vet Parasitol. 2013; 196:528-531.
• [31]Motta B, Nägeli F, Nägeli C, Solari-Basano F, Schiessl B, Deplazes P, Schnyder M. Epidemiology of the eye worm Thelazia callipaeda in cats from southern Switzerland. Vet Parasitol. 2014; 203:287-293.
• [32]Otranto D, Dantas-Torres F, Mallia E, DiGeronimo PM, Brianti E, Testini G, Traversa D, Lia RP. Thelazia callipaeda (Spirurida, Thelaziidae) in wild animals: report of new host species and ecological implications. Vet Parasitol. 2009; 166:262-267.
• [33]Calero-Bernal R, Otranto D, Pérez-Martín JE, Serrano FJ, Reina D. First report of Thelazia callipaeda in wildlife from Spain. J Wildl Dis. 2013; 49:458-460.
• [34]Sargo R, Loureiro F, Catarino AL, Valente J, Silva F, Cardoso L, Otranto D, Maia C. First report of Thelazia callipaeda in red foxes (Vulpes vulpes) from Portugal. J Zoo Wildl Med. 2014; 45:458-460.
• [35]Otranto D, Dantas-Torres F, Brianti E, Traversa D, Petrić D, Genchi C, Capelli G. Vector-borne helminths of dogs and humans in Europe. Parasit Vectors. 2013; 6:16. BioMed Central Full Text