Status of organophosphate and carbamate resistance in Anopheles gambiae sensu lato from the south and north Benin, West Africa

Particle and Fibre Toxicology

Martin Akogbéto²  Gil Germain Padonou²  Fiacre Agossa²  Olivier Oussou¹  Virgile Gnanguenon²  Rock Aïkpon²  Nazaire Aïzoun² 

[1] Centre de Recherche Entomologique de Cotonou (CREC), 06 BP 2604, Cotonou, Bénin;Faculté des Sciences et Techniques, Université d’Abomey Calavi, Calavi, Bénin

关键词: Benin;    IRS;    Bendiocarb;    Fenitrothion;    Resistance;    Ace-1;    Anopheles gambiae;   

Others  :  824546
DOI  :  10.1186/1756-3305-6-274

 received in 2013-06-08, accepted in 2013-09-17,  发布年份 2013

【 摘 要 】

Background

With the rapid spread of pyrethroid resistance in the main malaria vectors from Benin and the various resistance mechanisms involved (metabolic resistance and knock-down resistance (kdr), it is important to foresee effective resistance management strategies. Thus, the knowledge of the insensitive acetylcholinesterase (ace-1R) effects on phenotypes of An. gambiae will help us to strengthen basic and operational research on thedevelopment of strategies that will use organophosphates or carbamates as alternatives against pyrethroids-resistant malaria vectors in the field.

Methods

Larvae and pupae of Anopheles gambiae s.l. mosquitoes were collected from the breeding sites in Ouemé, Atacora, and Alibori departments. CDC susceptibility tests were conducted on unfed female mosquitoes aged 2–5 days old. CDC bioassays were performed with stock solutions of fenitrothion (50 μg per bottle) and bendiocarb (12.5 μg per bottle). PCR techniques were used to detect species and Ace-1 mutations.

Results

Anopheles gambiae Seme and Kandi populations were susceptible to fenitrothion whereas Anopheles gambiae Tanguieta and Malanville populations were resistant. An. gambiae populations from Seme, Kandi and Malanville were fully susceptible to bendiocarb whereas those from Tanguieta have developed a strong resistance to the same insecticide. A slight decrease in mortality rate was observed with 97.91% in populations of mosquitoes from Malanville. PCR revealed that all specimens tested were Anopheles gambiae s.s..

The presence of Ace-1R at very low frequency (0.01) was observed in Anopheles gambiae Malanville populations.

Conclusion

This study demonstrated the need to monitor organophosphate (OPs) and Carbamates resistance among populations of the An. gambiae s.l. in Benin, to determine its spread and anticipate vector control failure where these insecticides are used. However, further studies are needed to understand the current distribution of the Ace-1R mutation in other localities in the south-north transect Benin.

【 授权许可】

   
2013 Aïzoun et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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