Cytomolecular characterization of de novo formed rye B chromosome variants

Molecular Cytogenetics

Andreas Houben¹  Marcelo Guerra²  Sonja Klemme¹  André Marques² 

[1] Leibniz-Institute of Plant Genetics & Crop Plant Research (IPK), 06466, Gatersleben, Germany;Laboratory of Plant Cytogenetics and Molecular Biology, Department of Botany, UFPE, Recife, Brazil

关键词: B chromosome polymorphisms;    Rye;    Supernumerary chromosome;   

Others  :  1151296
DOI  :  10.1186/1755-8166-5-34

 received in 2012-06-27, accepted in 2012-06-28,  发布年份 2012

【 摘 要 】

Background

B chromosomes (Bs) are dispensable elements which occur in many species including rye (Secale cereale). We determined the organization of B variants to obtain insights into the origin of B polymorphisms in rye.

Results

The observed B variants were classified according to their morphology and in situ hybridization patterns with the B-specific repeats D1100 and CL11 into (I) long arm iso B, (II) D1100-deficient B and (III) small metacentric B variants. Long arm iso Bs are likely products of a meiotic centromere misdivision and subsequent duplication of the long arm, whereas small B variants are probably generated by chromosome breakage. Some deficient Bs experienced extensive amplification of CL11 repeats.

Conclusions

Both the pericentromere and the nondisjunction control region seem to be involved in the generation of rye B chromosome variants. However, due to the loss of the B-specific nondisjuction control region most of the variants generated are not capable to accumulate in a population.

【 授权许可】

   
2012 Marques et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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