Insecticidal and genotoxic activity of Psoralea corylifolia Linn. (Fabaceae) against Culex quinquefasciatus Say, 1823

Particle and Fibre Toxicology

Sandeep Kumar¹  Akhilesh C Pandey¹  Arvind Kumar²  Virendra K Dua¹ 

[1] National Institute of Malaria Research, Sector-3, Health Centre, Field Unit BHEL, Ranipur, Hardwar, Uttrakhand, 249403, India;Department of Biotechnology, Madhav Institute of Technology and Science, Gwalior, 474005 (M.P), India

关键词: GCMS;    Culex quinquefasciatus;    Psoralea corylifolia;    Essential oil;    DNA damage;    Genotoxicity;    Adulticidal activity;    Larvicidal activity;   

Others  :  1228073
DOI  :  10.1186/1756-3305-6-30

 received in 2012-08-31, accepted in 2012-12-27,  发布年份 2013

【 摘 要 】

Background

Indiscriminate use of synthetic insecticides to eradicate mosquitoes has caused physiological resistance. Plants provide a reservoir of biochemical compounds; among these compounds some have inhibitory effect on mosquitoes. In the present study the larvicidal, adulticidal and genotoxic activity of essential oil of Psoralea corylifolia Linn. against Culex quinquefasciatus Say was explored.

Methods

Essential oil was isolated from the seeds of P. corylifolia Linn. Larvicidal and adulticidal bioassay of Cx. quinquefasciatus was carried out by WHO method. Genotoxic activity of samples was determined by comet assay. Identification of different compounds was carried out by gas chromatography- mass spectrometry analysis.

Results

LC₅₀ and LC₉₀ values of essential oil were 63.38±6.30 and 99.02±16.63 ppm, respectively against Cx. quinquefasciatus larvae. The LD₅₀ and LD₉₀ values were 0.057±0.007 and 0.109±0.014 mg/cm² respectively against adult Cx. quinquefasciatus,. Genotoxicity of adults was determined at 0.034 and 0.069 mg/cm². The mean comet tail length was 6.2548±0.754 μm and 8.47±0.931 μm and the respective DNA damage was significant i.e. 6.713% and 8.864% in comparison to controls. GCMS analysis of essential oil revealed 20 compounds. The major eight compounds were caryophyllene oxide (40.79%), phenol,4-(3,7-dimethyl-3-ethenylocta-1,6-dienyl) (20.78%), caryophyllene (17.84%), α-humulene (2.15%), (+)- aromadendrene (1.57%), naphthalene, 1,2,3,4-tetra hydro-1,6-dimethyle-4-(1-methyl)-, (1S-cis) (1.53%), trans- caryophyllene (0.75%), and methyl hexadecanoate (0.67%).

Conclusion

Essential oil obtained from the seeds of P. corylifolia showed potent toxicity against larvae and adult Cx. quinquefasciatus. The present work revealed that the essential oil of P. corylifolia could be used as environmentally sound larvicidal and adulticidal agent for mosquito control.

【 授权许可】

   
2013 Dua et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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Figure 4.

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