Defensins from the tick Ixodes scapularis are effective against phytopathogenic fungi and the human bacterial pathogen Listeria grayi

Particle and Fibre Toxicology

Mohammad Rahnamaeian⁴  Libor Grubhoffer²  Andreas Vilcinskas⁴  Zdeněk Franta¹  Lesley Bell-Sakyi⁵  Radek Šíma⁶  Martin Strnad²  Tereza Chrudimská²  Ryan OM Rego⁶  James J Valdés⁶  Alejandro Cabezas-Cruz³  Miray Tonk² 

[1] Fraunhofer Institute for Molecular Biology and Applied Ecology, Department of Bioresources, Winchester Strasse, Giessen, D-35394, Germany;Faculty of Science, University of South Bohemia, Branišovská 31, České Budějovice, 37005, Czech Republic;Center for Infection and Immunity of Lille (CIIL), INSERM U1019 – CNRS UMR 8204, Université Lille Nord de France, Institut Pasteur de Lille, Lille, France;Institute for Phytopathology and Applied Zoology, Justus-Liebig-University of Giessen, Heinrich-Buff-Ring 26-32, Giessen, D-35392, Germany;The Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, UK;Biology Centre of the AS CR, Institute of Parasitology, Branišovská 31, České Budějovice, 37005, Czech Republic

关键词: Tick cell line;    Ixodes scapularis;    Fusarium spp;    Listeria grayi;    Defensin;    Antimicrobial peptide;   

Others  :  1148907
DOI  :  10.1186/s13071-014-0554-y

 received in 2014-05-19, accepted in 2014-11-21,  发布年份 2014

【 摘 要 】

Background

Ixodes scapularis is the most common tick species in North America and a vector of important pathogens that cause diseases in humans and animals including Lyme disease, anaplasmosis and babesiosis. Tick defensins have been identified as a new source of antimicrobial agents with putative medical applications due to their wide-ranging antimicrobial activities. Two multigene families of defensins were previously reported in I. scapularis. The objective of the present study was to characterise the potential antimicrobial activity of two defensins from I. scapularis with emphasis on human pathogenic bacterial strains and important phytopathogenic fungi.

Methods

Scapularisin-3 and Scapularisin-6 mature peptides were chemically synthesised. In vitro antimicrobial assays were performed to test the activity of these two defensins against species of different bacterial genera including Gram-positive bacteria Staphylococcus aureus, Staphylococcus epidermidis, and Listeria spp. as well as Gram-negative bacteria Escherichia coli, Pseudomonas aeruginosa along with two plant-pathogenic fungi from the genus Fusarium. In addition, the tissue-specific expression patterns of Scapularisin-3 and Scapularisin-6 in I. scapularis midgut, salivary glands and embryo-derived cell lines were determined using PCR. Finally, tertiary structures of the two defensins were predicted and structural analyses were conducted.

Results

Scapularisin-6 efficiently killed L. grayi, and both Scapularisin-3 and Scapularisin-6 caused strong inhibition (IC₅₀ value: ~1 μM) of the germination of plant-pathogenic fungi Fusarium culmorum and Fusarium graminearum. Scapularisin-6 gene expression was observed in I. scapularis salivary glands and midgut. However, Scapularisin-3 gene expression was only detected in the salivary glands. Transcripts from the two defensins were not found in the I. scapularis tick cell lines ISE6 and ISE18.

Conclusion

Our results have two main implications. Firstly, the anti-Listeria and antifungal activities of Scapularisin-3 and Scapularisin-6 suggest that these peptides may be useful for (i) treatment of antibiotic-resistant L. grayi in humans and (ii) plant protection. Secondly, the antimicrobial properties of the two defensins described in this study may pave the way for further studies regarding pathogen invasion and innate immunity in I. scapularis.

【 授权许可】

   
2014 Tonk et al.; licensee BioMed Central.

【 图 表 】

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