Tick capillary feeding for the study of proteins involved in tick-pathogen interactions as potential antigens for the control of tick infestation and pathogen infection

Particle and Fibre Toxicology

José de la Fuente⁵  Ana Domingos⁶  Pilar Alberdi⁴  José M Pérez de la Lastra⁴  Sabine Weisheit³  Rennos Fragkoudis³  Lesley Bell-Sakyi³  Juan A Moreno-Cid⁴  Juan Mosqueda²  Octavio Merino⁴  Sandra Antunes¹ 

[1] Instituto de Higiene e Medicina Tropical, Rua da Junqueira 100, 1349-008 Lisboa, Portugal;Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Campus Juriquilla CP 76230, Querétaro Mexico;The Pirbright Institute, Ash Road, Pirbright, Woking GU24 0NF UK;SaBio. Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain;Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA;Centro de Malária e Outras Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Rua da Junqueira 100 1349-008 Lisboa Portugal

关键词: Tick cell line;    Vaccine;    Capillary feeding;    Babesia;    Anaplasma;    Pathogen;    Tick;   

Others  :  814348
DOI  :  10.1186/1756-3305-7-42

 received in 2013-12-30, accepted in 2014-01-19,  发布年份 2014

【 摘 要 】

Background

Ticks represent a significant health risk to animals and humans due to the variety of pathogens they can transmit during feeding. The traditional use of chemicals to control ticks has serious drawbacks, including the selection of acaricide-resistant ticks and environmental contamination with chemical residues. Vaccination with the tick midgut antigen BM86 was shown to be a good alternative for cattle tick control. However, results vary considerably between tick species and geographic location. Therefore, new antigens are required for the development of vaccines controlling both tick infestations and pathogen infection/transmission. Tick proteins involved in tick-pathogen interactions may provide good candidate protective antigens for these vaccines, but appropriate screening procedures are needed to select the best candidates.

Methods

In this study, we selected proteins involved in tick-Anaplasma (Subolesin and SILK) and tick-Babesia (TROSPA) interactions and used in vitro capillary feeding to characterize their potential as antigens for the control of cattle tick infestations and infection with Anaplasma marginale and Babesia bigemina. Purified rabbit polyclonal antibodies were generated against recombinant SUB, SILK and TROSPA and added to uninfected or infected bovine blood to capillary-feed female Rhipicephalus (Boophilus) microplus ticks. Tick weight, oviposition and pathogen DNA levels were determined in treated and control ticks.

Results

The specificity of purified rabbit polyclonal antibodies against tick recombinant proteins was confirmed by Western blot and against native proteins in tick cell lines and tick tissues using immunofluorescence. Capillary-fed ticks ingested antibodies added to the blood meal and the effect of these antibodies on tick weight and oviposition was shown. However, no effect was observed on pathogen DNA levels.

Conclusions

These results highlighted the advantages and some of the disadvantages of in vitro tick capillary feeding for the characterization of candidate tick protective antigens. While an effect on tick weight and oviposition was observed, the effect on pathogen levels was not evident probably due to high tick-to-tick variations among other factors. Nevertheless, these results together with previous results of RNA interference functional studies suggest that these proteins are good candidate vaccine antigens for the control of R. microplus infestations and infection with A. marginale and B. bigemina.

【 授权许可】

   
2014 Antunes et al.; licensee BioMed Central Ltd.

【 图 表 】

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