Mini-FLOTAC, a new tool for copromicroscopic diagnosis of common intestinal nematodes in dogs

Particle and Fibre Toxicology

Giuseppe Cringoli³  Gerald C Coles¹  Paola Pepe³  Settimia Alfano²  Laura Rinaldi³  Maria P Maurelli³ 

[1] School of Veterinary Sciences, University of Bristol, Langford House, Bristol, UK;Department of Sperimental Medicine, Second University of Naples, Naples, Italy;Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, CREMOPAR Regione Campania, Via Della Veterinaria 1, 80137 Naples, Italy

关键词: Sensitivity;    Intestinal nematodes;    FLOTAC;    Mini-FLOTAC;    Wisconsin;    Flotation;    Dog;    Direct smear;   

Others  :  1181880
DOI  :  10.1186/1756-3305-7-356

 received in 2014-04-04, accepted in 2014-07-21,  发布年份 2014

【 摘 要 】

Background

The most common intestinal nematodes of dogs are Toxocara canis, hookworm and Trichuris vulpis. The present study was aimed to validate a new copromicroscopic technique, the Mini-FLOTAC and to compare its diagnostic efficiency and sensitivity with four other copromicroscopic techniques: direct smear, tube flotation, Wisconsin method and the FLOTAC dual technique.

Findings

Two experiments were performed. In the first, faecal positive samples collected from 59 stray asymptomatic dogs, of which 21 were naturally infected with ancylostomidae, 13 naturally infected with T. canis and 25 naturally infected with T. vulpis were used to validate the Mini-FLOTAC technique. The second experiment was performed on faecal samples randomly selected from 38 stray asymptomatic dogs to compare the diagnostic efficiency and sensitivity of the different techniques. Samples were fixed with 5% formalin; sodium chloride and zinc sulphate were used for flotation solutions because they performed best for detecting and quantifying intestinal nematode eggs in dogs. Mini-FLOTAC and FLOTAC were the most efficient and sensitive techniques and they gave higher EPG and higher numbers of positive samples in both the experiments, for all three parasites.

Conclusions

As Mini-FLOTAC does not require centrifugation it is a very promising technique for counting helminth eggs in dog faeces.

【 授权许可】

   
2014 Maurelli et al.; licensee BioMed Central Ltd.

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