Efficacy of a proprietary formulation of fipronil/(S)-methoprene/cyphenothrin against Ixodes scapularis tick infestations on dogs

Particle and Fibre Toxicology

Ruchika Solanki²  Sheila J. Gross³  William Russell Everett¹  Doug Carithers² 

[1] BerTek, Inc., Greenbrier, AR, USA;Merial, Inc., 3239 Satellite Blvd, Duluth 30096, GA, USA;Independent Contract Statistician, Piscataway, NJ, USA

关键词: Tick control;    Efficacy;    Dogs;    Deer tick;    Ixodes scapularis;   

Others  :  1222205
DOI  :  10.1186/s13071-015-0992-1

 received in 2015-05-15, accepted in 2015-07-08,  发布年份 2015

【 摘 要 】

Background

Efficacy of FRONTLINE® TRITAK® For Dogs (fipronil/(S)-methoprene/cyphenothrin, Merial, Inc., Duluth, GA) against Ixodes scapularis was evaluated in two separate, but concurrent laboratory studies.

Methods

One day after topical treatment with placebo or active, dogs (n = 24) were infested with 50 unfed adult Ixodes scapularis ticks, with repeat infestations on Days 7, 14, 21 and 28. The number of live ticks was counted at 6 hours post-infestation in the first study (n = 12) and at 24 hours post-infestation in the second study (n = 12).

Results

Observed efficacies in study 1 were 93-99 % at 6 hour assessments on Day 1 through Day 28 and in the second study, 98-100 % at 24 hour assessments, occurring on Day 2 through Day 29.

Conclusions

A single dose of FRONTLINE® TRITAK® For Dogs (fipronil/(S)-methoprene/cyphenothrin) (0.67 ml or 1.34 ml) prevented the establishment of a new infestation following treatment, as well as the repeated weekly re-infestations with Ixodes scapularis ticks, for 4 weeks.

【 授权许可】

   
2015 Carithers et al.

【 参考文献 】

• [1]Chomel B. Tick-borne infections in dogs—an emerging infectious threat. Vet Parasitol. 2011; 179(4):294-301.
• [2]Varde S, Beckley J, Schwartz I. Prevalence of tick-borne pathogens in Ixodes scapularis in a rural New Jersey County. Emerg Infect Dis. 1998; 4(1):97-99.
• [3]Dennis DT, Nekomoto TS, Victor JC, Paul WS, Piesman J. Reported distribution of Ixodes scapularis and Ixodes pacificus (Acari: Ixodidae) in the United States. J Med Entomol. 1998; 35(5):629-638.
• [4]Piesman J, Mather TN, Sinsky RJ, Spielman A. Duration of tick attachment and Borrelia burgdorferi transmission. J Clin Microbiol. 1987; 25(3):557-558.
• [5]Des Vignes F, Piesman J, Heffernan R, Schulze TL, Stafford KC, Fish D. Effect of tick removal on transmission of Borrelia burgdorferi and Ehrlichia phagocytophila by Ixodes scapularis nymphs. J Infect Dis. 2001; 183(5):773-778.
• [6]Piesman J, Dolan MC. Protection against Lyme disease spirochete transmission provided by prompt removal of nymphal Ixodes scapularis (Acari: Ixodidae). J Med Entomol. 2002; 39(3):509-512.
• [7]Greene C. Environmental factors in infectious disease. In: Infectious diseases of the dog and cat. Greene C, editor. Saunders Elsevier, St Louis, MI; 2006: p.991-1012.
• [8]Marchiondo AA, Holdsworth PA, Fourie LJ, Rugg D, Hellmann K, Snyder DE, Dryden MW. World Association for the Advancement of Veterinary Parasitology (WAAVP): guidelines for evaluating the efficacy of parasiticides for the treatment, prevention and control of flea and tick infestations on dogs and cats. Vet Parasitol. 2013; 194(1):84-97.
• [9]Dryden MW, Payne PA, Smith V, Hostetler J. Evaluation of an imidacloprid (8.8% w/w)-permethrin (44.0% w/w) topical spot-on and a fipronil (9.8% w/w)-(S)-methoprene (8.8% w/w) topical spot-on to repel, prevent attachment, and kill adult Ixodes scapularis and Amblyomma americanum ticks on dogs. Vet Ther. 2006; 7(3):173.
• [10]Efficacy of Activyl® Tick Plus for the treatment and control of various species of tick infestations on dogs. (2012). MAH-ACT-36 http://www.merck-animal-health-usa.com/binaries/ACT-36-Tick-Plus-Efficacy_tcm96-154693.pdf (accessed 15 June, 2015).
• [11]Jacobson R, McCall J, Hunter J, Alva R, Irwin J, Eschner A, Boeckh A. The ability of fipronil to prevent transmission of Borrelia burgdorferi, the causative agent of Lyme disease to dogs. JARVM. 2004; 2(1):39-45.
• [12]Bonneau S, Gupta S, Cadiergues MC. Comparative efficacy of two fipronil spot-on formulations against experimental tick infestations (Ixodes ricinus) in dogs. Parasitol Res. 2010; 107(3):735-739.