Absence of genetic structure in Baylisascaris schroederi populations, a giant panda parasite, determined by mitochondrial sequencing

Particle and Fibre Toxicology

Guangyou Yang¹  Xuerong Peng⁴  Tao Wang¹  Xiaobin Gu¹  Tianyu Liu¹  Yun Sun¹  Chengdong Wang³  Zhihe Zhang³  Xuan Zhou²  Yue Xie¹ 

[1] Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, China;Centre for Animal Diseases Control and Prevention, Dachuan Animal Husbandry Bureau, Dazhou 623000, China;Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China;Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya’an 625014, China

关键词: Mitochondrial cox1;    Mitochondrial atp6;    Genetic diversity;    Roundworm;    Ailuropoda melanoleuca;   

Others  :  1148188
DOI  :  10.1186/s13071-014-0606-3

 received in 2014-11-11, accepted in 2014-12-16,  发布年份 2014

【 摘 要 】

Background

Infection with the parasitic nematode, Baylisascaris schroederi (Ascaridida: Nematoda), is one of the most important causes of death in giant pandas, and was responsible for half of deaths between 2001 and 2005. Mitochondrial (mt) DNA sequences of parasites can unveil their genetic diversity and depict their likely dynamic evolution and therefore may provide insights into parasite survival and responses to host changes, as well as parasite control.

Methods

Based on previous studies, the present study further annotated the genetic variability and structure of B. schroederi populations by combining two different mtDNA markers, ATPase subunit 6 (atp6) and cytochrome c oxidase subunit I (cox1). Both sequences were completely amplified and genetically analyzed among 57 B. schroederi isolates, which were individually collected from ten geographical regions located in three important giant panda habitats in China (Minshan, Qionglai and Qinling mountain ranges).

Results

For the DNA dataset, we identified 20 haplotypes of atp6, 24 haplotypes of cox1, and 39 haplotypes of atp6 + cox1. Further haplotype network and phylogenetic analyses demonstrated that B. schroederi populations were predominantly driven by three common haplotypes, atp6 A1, cox1 C10, and atp6 + cox1 H11. However, due to low rates of gene differentiation between the three populations, both the atp6 and cox1 genes appeared not to be significantly associated with geographical divisions. In addition, high gene flow was detected among the B. schroederi populations, consistent with previous studies, suggesting that this parasite may be essentially homogenous across endemic areas. Finally, neutrality tests and mismatch analysis indicated that B. schroederi had undergone earlier demographic expansion.

Conclusions

These results confirmed that B. schroederi populations do not follow a pattern of isolation by distance, further revealing the possible existence of physical connections before geographic separation. This study should also contribute to an improved understanding of the population genetics and evolutionary biology of B. schroederi and assist in the control of baylisascariasis in giant pandas.

【 授权许可】

   
2014 Xie et al.; licensee BioMed Central.

【 图 表 】

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