Adaptation of Salmonella enterica Hadar under static magnetic field: effects on outer membrane protein pattern

Proteome Science

Emmanuelle Dɹ  Ahmed Landoulsi²  Thierry Jouenne¹  Philippe Chan¹  Laurent Coquet¹  Alya El May²  Sarra Snoussi² 

[1] UMR 6270 CNRS, Faculté des Sciences, Université de Rouen, 76821, Mont Saint Aignan Cedex, France;Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences de Bizerte, Université de Carthage, Zarzouna, Bizerte, Tunisie

关键词: Outer membrane proteome;    Static magnetic field;    Salmonella;   

Others  :  817618
DOI  :  10.1186/1477-5956-10-6

 received in 2011-08-11, accepted in 2012-02-03,  发布年份 2012

【 摘 要 】

Background

Salmonella enterica serovar Hadar (S. Hadar) is a highly prevalent foodborne pathogen and therefore a major cause of human gastroenteritis worldwide. Outer membrane proteins whose production is often regulated by environmental conditions also play important roles in the adaptability of bacterial pathogens to various environments.

Results

The present study investigated the adaptation of S. Hadar under the effect of acute static magnetic field exposure (200 mT, 9 h) and the impact on the outer membrane protein pattern. Via two-dimensional electrophoresis (2-DE) and LC-MS/MS spectrometry, we compared the proteome of enriched-outer membrane fraction before and after exposure to a magnetic field. A total of 11 proteins, displaying more than a two-fold change, were differentially expressed in exposed cells, among which 7 were up-regulated and 4 down-regulated. These proteins were involved in the integrity of cell envelope (TolB, Pal), in the response to oxidative stress (OmpW, dihydrolipoamide dehydrogenase, UspF), in the oxidative stress status (bacterioferritin), in virulence (OmpX, Yfgl) or in motility (FlgE and UspF). Complementary experiments associated the down-regulation of FlgE and UspF with an alteration of swarming, a flagella-driven motility, under SMF. Furthermore, the antibiotic disc diffusion method confirmed a decrease of gentamicin susceptibility in exposed cells. This decrease could be partly associated with the up-regulation of TolC, outer membrane component of an efflux pump. OmpA, a multifunctional protein, was up-regulated.

Conclusions

SMF (200 mT) seems to maintain the cell envelope integrity and to submit the exposed cells to an oxidative stress. Some alterations suggest an increase of the ability of exposed cells to form biofilms.

【 授权许可】

   
2012 Snoussi et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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