Advantage of using colonic washouts for Blastocystis detection in colorectal cancer patients

Particle and Fibre Toxicology

Suresh Kumar Govind³  Kuppusamy Umah Rani²  April Camilla Roslani¹  Vinoth Kumarasamy³ 

[1] Department of Surgery, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia;Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia;Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia

关键词: Subtypes;    Blastocystis;    Colorectal cancer;   

Others  :  807401
DOI  :  10.1186/1756-3305-7-162

 received in 2014-01-17, accepted in 2014-03-12,  发布年份 2014

【 摘 要 】

Background

There have been previous studies associating microorganisms to cancer and with our recent findings of Blastocytsis antigen having a higher in vitro proliferation of cancer cells strengthens the suspicion. Collecting faecal samples alone to associate this parasite with cancer may not be accurate due to the phenomenon of irregular shedding and the possible treatment administrated to the cancer patients. Hence, this become the basis to search for an alternate method of sample collection. Colonic washout is an almost complete washed up material from colon and rectum which includes various microorganisms such as Blastocystis and other lodged material within the villi. The detection of parasite in colonic washouts will give a better reflection on the association between Blastocystis and CRC.

Methods

Blastocytsis detection was made by in vitro culture method using Jones’ medium, formal ether concentration technique and conventional polymerase chain reaction (PCR) on faecal samples and colonic washouts of 204 CRC patients from colonoscopy procedure. Faecal samples and colonic washouts from 221 normal individuals served as control.

Results

We observed an increased detection of Blastocystis using colonic washouts (n = 53, 12.47%) than faecal samples (n = 26, 6.12%). Eleven faecal samples showed positive results for Blastocystis which were also found in colonic washouts using the PCR technique. This study for the first time showed a significant Blastocystis infection among CRC patients (n = 43, 21.08%) compared to the asymptomatic normal individuals (n = 22, 9.95%). Blastocystis subtype 3 infection was found to be significantly more prevalent (n = 26, 12.75%) compared to other subtypes namely subtype 1: n = 9 (4.41%), subtype 2: n = 1 (0.49%), subtype 5: n = 1 (0.49%) and mixed subtype: n = 6 (2.94%) among the CRC patients.

Conclusion

The study showed that colonic washouts provide a better alternative for Blastocystis detection in CRC patients compared to faecal samples as this prevents treatment regime and the phenomenon of irregular shedding from influencing the detection results obtained from faecal samples.

【 授权许可】

   
2014 Kumarasamy et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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