First detailed reconstruction of the karyotype of Trachypithecus cristatus (Mammalia: Cercopithecidae)

Molecular Cytogenetics

Anja Weise³  Marcelo de Bello Cioffi²  Thomas Liehr⁷  Arunrat Chaveerach¹  Alongkoad Tanomtong¹  Weerayuth Supiwong¹  Nadezda Kosyakova⁷  Pornnarong Siripiyasing⁶  Hasmik Mkrtchyan⁵  Krit Pinthong⁴  Fan Xiaobo⁷ 

[1] Department of Biology, Faculty of Science, Khon Kaen University, 123 Moo 16 Mittapap Rd., Khon Kaen, Muang District 40002, Thailand;Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brazil;Institut für Humangenetik, Postfach, Jena D-07740, Germany;Faculty of Science and Technology, Surindra Rajabhat University, 186 Moo 1, Surin, Maung District 32000, Thailand;Center of Medical Genetics and Primary Health Care, Abovyan Str 34/3, 001, Yerevan, Armenia;Faculty of Science and Technology, Rajabhat Maha Sarakham University, 80 Nakonsawan Rd, Maha Sarakham, Talad, Maung District 44000, Thailand;Institute of Human Genetics, Jena University Hospital, Friedrich Schiller University, Kollegiengasse 10, Jena D-07743, Germany

关键词: XY₁Y₂ sex system;    Old world monkeys;    Multicolor banding;    Evolutionary conserved breakpoints;   

Others  :  1150522
DOI  :  10.1186/1755-8166-6-58

 received in 2013-11-15, accepted in 2013-11-21,  发布年份 2013

【 摘 要 】

Background

The chromosomal homologies of human (Homo sapiens = HSA) and silvered leaf monkey (Trachypithecus cristatus = TCR) have been previously studied by classical chromosome staining and by fluorescence in situ hybridization (FISH) applying chromosome-specific DNA probes of all human chromosomes in the 1980s and 1990s, respectively.

Results

However, as the resolution of these techniques is limited we used multicolor banding (MCB) at an ~250-band level, and other selected human DNA probes to establish a detailed chromosomal map of TCR. Therefore it was possible to precisely determine evolutionary conserved breakpoints, orientation of segments and distribution of specific regions in TCR compared to HSA. Overall, 69 evolutionary conserved breakpoints including chromosomal segments, which failed to be resolved in previous reports, were exactly identified and characterized.

Conclusions

This work also represents the first molecular cytogenetic one characterizing a multiple sex chromosome system with a male karyotype 44,XY₁Y₂. The obtained results are compared to other available data for old world monkeys and drawbacks in hominoid evolution are discussed.

【 授权许可】

   
2013 Xiaobo et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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