The interferon-inducible antiviral protein Daxx is not essential for interferon-mediated protection against avian sarcoma virus

Virology Journal

Siddharth Balachandran¹  Richard A Katz¹  Anna Marie Skalka¹  Shoko Nogusa¹  Natalia Shalginskikh¹  Kelsey A Haugh¹ 

[1] Immune Cell Development and Host Defense Program, Fox Chase Cancer Center, Room 422 Reimann Building, 333 Cottman Ave., 19111 Philadelphia, PA, USA

关键词: Innate immunity;    Avian sarcoma virus;    Interferon;    Daxx;   

Others  :  804053
DOI  :  10.1186/1743-422X-11-100

 received in 2014-02-03, accepted in 2014-05-23,  发布年份 2014

【 摘 要 】

Background

The antiviral protein Daxx acts as a restriction factor of avian sarcoma virus (ASV; Retroviridae) in mammalian cells by promoting epigenetic silencing of integrated proviral DNA. Although Daxx is encoded by a type I (α/β) interferon-stimulated gene, the requirement for Daxx in the interferon anti-retroviral response has not been elucidated. In this report, we describe the results of experiments designed to investigate the role of Daxx in the type I interferon-induced anti-ASV response.

Findings

Using an ASV reporter system, we show that type I interferons are potent inhibitors of ASV replication. We demonstrate that, while Daxx is necessary to silence ASV gene expression in the absence of interferons, type I interferons are fully-capable of inducing an antiviral state in the absence of Daxx.

Conclusions

These results provide evidence that Daxx is not essential for the anti-ASV interferon response in mammalian cells, and that interferons deploy multiple, redundant antiviral mechanisms to protect cells from ASV.

【 授权许可】

   
2014 Haugh et al.; licensee BioMed Central Ltd.

【 图 表 】

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