The combination matters - distinct impact of lifestyle factors on sperm quality: a study on semen analysis of 1683 patients according to MSOME criteria

Reproductive Biology and Endocrinology

Nicolas H Zech¹  Birgit Schechinger¹  Maximilian Schuff¹  Dietmar Spitzer²  Anton Neyer¹  Pierre Vanderzwalmen³  Astrid Stecher¹  Barbara Wirleitner¹  Johannes Wogatzky¹ 

[1] IVF Centers Prof Zech-Bregenz, Roemerstrasse 2, Bregenz, 6900, Austria;IVF Centers Prof Zech-Salzburg, Innsbrucker Bundesstr. 35, Salzburg, 5020, Austria;Centre Hospitalier Inter Régional Cavell (CHIREC), 1420 Braine-l‘alleud, Brussels, Belgium

关键词: Oxidative stress;    Ejaculation frequency;    BMI;    Lifestyle;    IMSI;    MSOME;    Sperm quality;   

Others  :  829479
DOI  :  10.1186/1477-7827-10-115

 received in 2012-10-08, accepted in 2012-12-12,  发布年份 2012

【 摘 要 】

Background

Poor sperm quality can negatively affect embryonic development and IVF outcome. This study is aimed at investigating the influence of various lifestyle factors on semen quality according to MSOME (motile sperm organelle morphology examination) criteria.

Methods

1683 male patients undergoing assisted reproductive technologies (ART) in our clinic were surveyed about their age, BMI (body mass index), ejaculation frequency, nutrition, sports, sleeping habits and social behavior. Semen samples were collected and evaluation of semen parameters according to MSOME and WHO criteria was performed. Results were grouped and statistically analyzed.

Results

Although single parameters had minor effects on sperm parameter, the combination of age, BMI, coffee intake, ejaculatory frequency and duration of sexual abstinence were identified as factors having a negative effect on sperm motility. Additionally, we could demonstrate that MSOME quality was reduced. The negative impact of age, BMI and coffee intake on sperm quality could be compensated if patients had a high ejaculation frequency and shorter periods of sexual abstinence.

Conclusions

Combinations of adverse lifestyle factors could have a detrimental impact on sperm, not only in terms of motility and sperm count but also in terms of sperm head vacuolization. This negative impact was shown to be compensated by higher ejaculation frequency and a shorter period of sexual abstinence. The compensation is most likely due to a shorter storage time in the male gonads, thus reducing the duration of sperms’ exposure to reactive oxygen species (ROS).

【 授权许可】

   
2012 Wogatzky et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Braga DP, Halpern G, Figueira Rde C, Setti AS, Iaconelli A Jr, Borges E Jr: Food intake and social habits in male patients and its relationship to intracytoplasmic sperm injection outcomes. Fertil Steril 2012, 97:53-59.
• [2]Sekhavat L, Moein MR: The effect of male body mass index on sperm parameters. Aging Male 2010, 13:155-158.
• [3]Gaur DS, Talekar MS, Pathak VP: Alcohol intake and cigarette smoking: impact of two major lifestyle factors on male fertility. Indian J Pathol Microbiol 2010, 53:35-40.
• [4]Jensen TK, Swan SH, Skakkebaek NE, Rasmussen S, Jørgensen N: Caffeine intake and semen quality in a population of 2,554 young danish men. Am J Epidemiol 2010, 171:883-891.
• [5]Jensen TK, Andersson AM, Jørgensen N, Andersen AG, Carlsen E, Petersen JH, Skakkebaek NE: Body mass index in relation to semen quality and reproductive hormones among 1,558 danish men. Fertil Steril 2004, 82:863-870.
• [6]Kruger TF, Acosta AA, Simmons KF, Swanson RJ, Matta JF, Veeck LL, Morshedi M, Brugo S: New method of evaluating sperm morphology with predictive value for human in vitro fertilization. Arch Androl 1987, 18:275-277.
• [7]Menkveld R, Kruger TF: Advantages of strict (tygerberg) criteria for evaluation of sperm morphology. Int J Androl 1995, 18:36-42. Review
• [8]World Health Organization: WHO Laboratory Manual for the Examination of Human Semen and Sperm-cervical Mucus Interaction. 3rd edition. Cambridge University Press, Cambridge; 1992:107p.
• [9]World Health Organisation: Department of Reproductive Health and Research WHO laboratory manual for the examination and processing of human semen. 5th edition. 2010, 287p.
• [10]Bartoov B, Berkovitz A, Eltes F, Kogosowski A, Menezo Y, Barak Y: Real-time fine morphology of motile human sperm cells is associated with IVF-ICSI outcome. J Androl 2002, 23:1-8.
• [11]Berkovitz A, Eltes F, Soffer Y, Zabludovsky N, Beyth Y, Farhi J, Levran D, Bartoov B: ART success and in vivo sperm cell selection depend on the ultramorphological status of spermatozoa. Andrologia 1999, 31:1-8.
• [12]Vanderzwalmen P, Hiemer A, Rubner P, Bach M, Neyer A, Stecher A, Uher P, Zintz M, Lejeune B, Vanderzwalmen S, et al.: Blastocyst development after sperm selection at high magnification is associated with size and number of nuclear vacuoles. Reprod Biomed Online 2008, 17:617-627.
• [13]Berkovitz A, Eltes F, Lederman H, Peer S, Ellenbogen A, Feldberg B, Bartoov B: How to improve IVF-ICSI outcome by sperm selection. Reprod Biomed Online 2006, 12:634-638.
• [14]Hazout A, Dumont-Hassan M, Junca AM, Cohen Bacrie P, Tesarik J: High-magnification ICSI overcomes paternal effect resistant to conventional ICSI. Reprod Biomed Online 2006, 12:19-25.
• [15]Antinori M, Licata E, Dani G, Cerusico F, Versaci C, d'Angelo D, Antinori S: Intracytoplasmic morphologically selected sperm injection: a prospective randomized trial. Reprod Biomed Online 2008, 16:835-841.
• [16]Nadalini M, Tarozzi N, Distratis V, Scaravelli G, Borini A: Impact of intracytoplasmic morphologically selected sperm injection on assisted reproduction outcome: a review. Reprod Biomed Online 2009, 19:45-55. Review
• [17]Souza Setti A, Ferreira RC, Paes de Almeida Ferreira Braga D, de Cássia Sávio Figueira R, Iaconelli A Jr, Borges E Jr: Intracytoplasmic sperm injection outcome versus intracytoplasmic morphologically selected sperm injection outcome: a meta-analysis. Reprod Biomed Online 2010, 21:450-455. Review
• [18]Knez K, Zorn B, Tomazevic T, Vrtacnik-Bokal E, Virant-Klun I: The IMSI procedure improves poor embryo development in the same infertile couples with poor semen quality: a comparative prospective randomized study. Reprod Biol Endocrinol 2011, 9:123. BioMed Central Full Text
• [19]Oliveira JB, Petersen CG, Massaro FC, Baruffi RL, Mauri AL, Silva LF, Ricci J, Franco JG Jr: Motile sperm organelle morphology examination (MSOME): intervariation study of normal sperm and sperm with large nuclear vacuoles. Reprod Biol Endocrinol 2010, 8:56. BioMed Central Full Text
• [20]Hammoud I, Boitrelle F, Ferfouri F, Vialard F, Bergere M, Wainer B, Bailly M, Albert M, Selva J: Selection of normal spermatozoa with a vacuole-free head (x6300) improves selection of spermatozoa with intact DNA in patients with high sperm DNA fragmentation rates. Andrologia 2012., 26
• [21]Boitrelle F, Ferfouri F, Petit JM, Segretain D, Tourain C, Bergere M, Bailly M, Vialard F, Albert M, Selva J: Large human sperm vacuoles observed in motile spermatozoa under high magnification: nuclear thumbprints linked to failure of chromatin condensation. Hum Reprod 2011, 26:1650-1658.
• [22]Franco JG Jr, Mauri AL, Petersen CG, Massaro FC, Silva LF, Felipe V, Cavagna M, Pontes A, Baruffi RL, Oliveira JB, et al.: Large nuclear vacuoles are indicative of abnormal chromatin packaging in human spermatozoa. Int J Androl 2012, 35:46-51.
• [23]Skowronek F, Casanova G, Alciaturi J, Capurro A, Cantu L, Montes JM, Sapiro R: DNA sperm damage correlates with nuclear ultrastructural sperm defects in teratozoospermic men. Andrologia 2012, 44:59-65.
• [24]Vanderzwalmen P, Bertin-Segal G, Geerts L, Debauche C, Schoysman R: Sperm morphology and IVF pregnancy rate: comparison between percoll gradient centrifugation and swim-up procedures. Hum Reprod 1991, 6:581-588.
• [25]Tesarik J, Greco E, Mendoza C: Late, but not early, paternal effect on human embryo development is related to sperm DNA fragmentation. Hum Reprod 2004, 19:611-615.
• [26]Zini A, Libman J: Sperm DNA damage: importance in the era of assisted reproduction. CMAJ 2006, 175:495-500. Review
• [27]World Health Organization: Obesity: preventing and managing the global epidemic. Report of a WHO consultation. World Health Organ Tech Rep Ser 2000, 894:1-253.
• [28]Aggerholm AS, Thulstrup AM, Toft G, Ramlau-Hansen CH, Bonde JP: Is overweight a risk factor for reduced semen quality and altered serum sex hormone profile? Fertil Steril 2008, 90:619-626.
• [29]Wegner CC, Clifford AL, Jilbert PM, Henry MA, Gentry WL: Abnormally high body mass index and tobacco use are associated with poor sperm quality as revealed by reduced sperm binding to hyaluronan-coated slides. Fertil Steril 2010, 93:332-334.
• [30]Qin DD, Yuan W, Zhou WJ, Cui YQ, Wu JQ, Gao ES: Do reproductive hormones explain the association between body mass index and semen quality? Asian J Androl 2007, 9:827-834.
• [31]Silva LF, Oliveira JB, Petersen CG, Mauri AL, Massaro FC, Cavagna M, Baruffi RL, Franco JG Jr: The effects of male age on sperm analysis by motile sperm organelle morphology examination (MSOME). Reprod Biol Endocrinol 2012, 19:10-19.
• [32]Hubert A, Szöke A, Leboyer M, Schürhoff F: Influence of paternal age in schizophrenia. Encéphale 2011, 37:199-206. Review
• [33]Reichenberg A, Gross R, Sandin S, Susser ES: Advancing paternal and maternal age are both important for autism risk. Am J Public Health 2010, 100:772-773.
• [34]Frans EM, Sandin S, Reichenberg A, Lichtenstein P, Långström N, Hultman CM: Advancing paternal age and bipolar disorder. Arch Gen Psychiatry 2008, 65:1034-1040.
• [35]Saha S, Barnett AG, Foldi C, Burne TH, Eyles DW, Buka SL, McGrath JJ: Advanced paternal age is associated with impaired neurocognitive outcomes during infancy and childhood. PLoS Med 2009, 6:e40.
• [36]Oldereid NB, Rui H, Purvis K: Life styles of men in barren couples and their relationship to sperm quality. Int J Fertil 1992, 37:343-349.
• [37]Mitra A, Chakraborty B, Mukhopadhay D, Pal M, Mukherjee S, Banerjee S, Chaudhuri K: Effect of smoking on semen quality, FSH, testosterone level, and CAG repeat length in androgen receptor gene of infertile men in an indian city. Syst Biol Reprod Med 2012, 58:255-62.
• [38]Selit I, Basha M, Maraee A, El-Naby SH, Nazeef N, El-Mehrath R, Mostafa T: Sperm DNA and RNA abnormalities in fertile and oligoasthenoteratozoospermic smokers. Andrologia 2012.
• [39]Saleh RA, Agarwal A, Sharma RK, Nelson DR, Thomas AJ Jr: Effect of cigarette smoking on levels of seminal oxidative stress in infertile men: a prospective study. Fertil Steril 2002, 78:491-499.
• [40]Hjollund NH, Bonde JP, Henriksen TB, Giwercman A, Olsen J: Danish first pregnancy planner study team. Reproductive effects of male psychologic stress. Epidemiology 2004, 15:21-27.
• [41]Hjollund NH, Bonde JP, Henriksen TB, Giwercman A, Olsen J: Danish first pregnancy planner study team. Job strain and male fertility. Epidemiology 2004, 15:114-117.
• [42]Eskiocak S, Gozen AS, Taskiran A, Kilic AS, Eskiocak M, Gulen S: Association between mental stress & some antioxidant enzymes of seminal plasma. Indian J Med Res 2005, 122:491-496.
• [43]Eskiocak S, Gozen AS, Kilic AS, Molla S: Effect of psychological stress on the L-arginine-nitric oxide pathway and semen quality. Braz J Med Biol Res 2006, 39:581-588.
• [44]Kennaway DJ, Boden MJ, Varcoe TJ: Circadian rhythms and fertility. Mol Cell Endocrinol 2012, 349:56-61.
• [45]Schnall MD: Electronmicroscopic study of human spermatozoa. Fertil Steril 1952, 3:62-82.
• [46]Brassesco M, Lopez G, Monqaut A, Lafuente R: Sperm vacuole improvement after antioxidant therapy. Fertil Steril 2011, 96:159.
• [47]Makker K, Agarwal A, Sharma R: Oxidative stress & male infertility. Indian J Med Res 2009, 129:357-367. Review
• [48]Showell MG, Brown J, Yazdani A, Stankiewicz MT, Hart RJ: Antioxidants formale subfertility. Cochrane Database Syst Rev 2011, (1):CD007411. Review
• [49]Mistry HD, Broughton Pipkin F, Redman CW, Poston L: Selenium in reproductive health. Am J Obstet Gynecol 2012, 206:21-30. Review
• [50]Moslemi MK, Tavanbakhsh S: Selenium-vitamin E supplementation in infertile men: effects on semen parameters and pregnancy rate. Int J Gen Med 2011, 4:99-104.
• [51]Oliva A, Dotta A, Multigner L: Pentoxifylline and antioxidants improve sperm quality in male patients with varicocele. Fertil Steril 2009, 91:1536-1539.
• [52]Schmid TE, Eskenazi B, Marchetti F, Young S, Weldon RH, Baumgartner A, Anderson D, Wyrobek AJ: Micronutrients intake is associated with improved sperm DNA quality in older men. Fertil Steril 2012, 98:1130-1137.