Genomic instability in complicated and uncomplicated Egyptian schistosomiasis haematobium patients

Molecular Cytogenetics

Tarek M Diab²  Rabab M Zalat²  Manal A Badawi⁴  Magda S Abd El-Tawab³  Ashraf M Emran⁵  Asmaa A Abd El-Aal¹  Maha M A Basyoni³  Ibrahim R Bayoumy²  Amany A Abd El-Aal³ 

[1]Clinical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

[2]Parasitology Department, Theodor Bilharz Research Institute, Giza, Egypt

[3]Parasitology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

[4]Pathology Department, National Research Center, Giza, Egypt

[5]Urosurgery Department, Faculty of Medicine, Cairo University, Cairo, Egypt

关键词: Karyotyping;    FISH;    Morphocytometry;    Chromosomal abnormalities;    Schistosomiasis haematobium;   

Others  :  1139697
DOI  :  10.1186/s13039-014-0104-5

 received in 2014-10-16, accepted in 2014-12-22,  发布年份 2015

【 摘 要 】

Background

Exploration of genetic changes during active Schistosoma infection is important for anticipation and prevention of chronic sequelae. This study aimed to explore the genomic instability in chromosomal and cellular kinetics in Egyptians suffering from uncomplicated active schistosomiasis haematobium infection in addition to chronic schistosomiasis haematobium cases complicated by bilharzial-associated bladder cancer (BAC).

Results

This study was conducted on 46 schistosomiasis haemotobium cases, 22 were active (Viable S. haematobium eggs in urine samples as detected by microscopy) and 24 were chronic complicated with bladder cancer. Three cytogenetic techniques were applied; the first was quantitative nuclear-morphocytometry by means of which the Feulgen-stained nuclei were analyzed for parameters including shape, size, integrated optical-density and nuclear area. The second was Fluorescent In-Situ Hybridization (FISH) for specific p53gene-locus of chromosome 17 and the third technique was karyotyping.

Concerning chronic complicated cases, the mean ± SD of DNA-content in urinary bladder tissue sections was 3.18 ± 0.65. Five samples (20.83%) of bladder tissue sections of chronic complicated cases showed diploid nuclei, 6 urinary bladder tissue samples (25%) were tetraploid, while 13 bladder samples (54.16%) were aneuploid. Epithelial cells of urine samples demonstrated aneuploidy (mean ± SD = 3.74 ± 0.36).Nuclear contents showed high proliferative DNA index in all urinary epithelial cells. In the acute uncomplicated group, nuclear-DNA of urinary epithelial cells was found diploid with mean nuclear-DNA content of 2.2 ± 0.16SD. Half of these diploid smears had a high proliferation index. The difference between nuclear DNA-contents in acute and chronic cases was significant (P = 0.0001). FISH technique for specific p53gene-locus and karyotyping were done on urinary bladder tissue specimens and peripheral blood monocytes of 8 chronic cases respectively. Three samples (37.5%) with invasive BAC had a deletion of the p53 gene. Karyotyping showed three cases out of the 8 chronic schistosomiasis haematobium patients with chromosomal fragmentations.

Conclusions

DNA morphometry was valuable in detection of gross genetic changes in urothelial tissues. It is an important prognostic factor in established schistosomiasis haematobium induced bladder malignancy. It has the great advantage of being applicable on urine cells making it suitable for the prediction of a tendency towards genetic instability in active schistosomiasis haematobium patients.

【 授权许可】

   
2015 Abdel Aal et al.; licensee BioMed Central.

【 图 表 】

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