Genome and proteome analysis of 7-7-1, a flagellotropic phage infecting Agrobacterium sp H13-3

Virology Journal

Rüdiger Schmitt²  Patrick Babinger⁴  Jean-Paul Noben⁵  Rob Lavigne³  An Van den Bossche³  Andrew M Kropinski¹ 

[1] Department of Molecular & Cellular Biology, University of Guelph, Guelph, NIG 2W1, ON, Canada;Institut für Biochemie, Genetik und Mikrobiologie, Universität Regensburg, D-93040, Regensburg, Germany;Division of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, 3001, Heverlee, Belgium;Institut für Biophysik und physikalische Biochemie, Universität Regensburg, D-93040, Regensburg, Germany;Biomedical Research Institute and Transnational University Limburg, School of Life Sciences, Hasselt University, Diepenbeek, Belgium

关键词: Posttranslational modification;    Bioinformatics;    Complex flagellum;    Proteome;    Genome;    Phage ecology;    Phage evolution;    Agrobacterium;   

Others  :  1154623
DOI  :  10.1186/1743-422X-9-102

 received in 2012-01-03, accepted in 2012-05-04,  发布年份 2012

【 摘 要 】

Background

The flagellotropic phage 7-7-1 infects motile cells of Agrobacterium sp H13-3 by attaching to and traveling along the rotating flagellar filament to the secondary receptor at the base, where it injects its DNA into the host cell. Here we describe the complete genomic sequence of 69,391 base pairs of this unusual bacteriophage.

Methods

The sequence of the 7-7-1 genome was determined by pyro(454)sequencing to a coverage of 378-fold. It was annotated using MyRAST and a variety of internet resources. The structural proteome was analyzed by SDS-PAGE coupled electrospray ionization-tandem mass spectrometry (MS/MS).

Results

Sequence annotation and a structural proteome analysis revealed 127 open reading frames, 84 of which are unique. In six cases 7-7-1 proteins showed sequence similarity to proteins from the virulent Burkholderia myovirus BcepB1A. Unique features of the 7-7-1 genome are the physical separation of the genes encoding the small (orf100) and large (orf112) subunits of the DNA packaging complex and the apparent lack of a holin-lysin cassette. Proteomic analysis revealed the presence of 24 structural proteins, five of which were identified as baseplate (orf7), putative tail fibre (orf102), portal (orf113), major capsid (orf115) and tail sheath (orf126) proteins. In the latter case, the N-terminus was removed during capsid maturation, probably by a putative prohead protease (orf114).

【 授权许可】

   
2012 Kropinski et al.; licensee BioMed Central Ltd.

【 图 表 】

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