期刊论文详细信息
Respiratory Research
Effects of different mesenchymal stromal cell sources and delivery routes in experimental emphysema
Patricia RM Rocco5  Marcelo M Morales2  Daniel J Weiss3  Débora G Xisto2  Vera L Capelozzi8  Nazareth N Rocha7  Isalira PRG Freitas4  Christina M Takyia6  Bruno L Diaz1  Priscilla C Olsen5  Elga Bandeira2  Miquéias Lopes-Pacheco2  Ana Clara Teixeira5  Fernanda F Cruz5  Soraia C Abreu5  Mariana A Antunes5 
[1] Laboratory of Inflammation, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil;Laboratory of Cellular and Molecular Physiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil;Department of Medicine, University of Vermont, Vermont, USA;Laboratory of Cellular and Molecular Cardiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil;Laboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Centro de Ciências da Saúde, Federal University of Rio de Janeiro, Avenida Carlos Chagas Filho, s/n, Bloco G-014, Ilha do Fundão – 21941-902, Rio de Janeiro, RJ, Brazil;Laboratory of Cellular Pathology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil;Fluminense Federal University, Niteroi, Rio de Janeiro, Brazil;Department of Pathology, University of São Paulo, São Paulo, Brazil
关键词: Mesenchymal stromal cells;    Macrophage;    Remodeling;    Emphysema;    Elastase;   
Others  :  1137260
DOI  :  10.1186/s12931-014-0118-x
 received in 2014-07-20, accepted in 2014-09-25,  发布年份 2014
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【 摘 要 】

We sought to assess whether the effects of mesenchymal stromal cells (MSC) on lung inflammation and remodeling in experimental emphysema would differ according to MSC source and administration route. Emphysema was induced in C57BL/6 mice by intratracheal (IT) administration of porcine pancreatic elastase (0.1 UI) weekly for 1 month. After the last elastase instillation, saline or MSCs (1×105), isolated from either mouse bone marrow (BM), adipose tissue (AD) or lung tissue (L), were administered intravenously (IV) or IT. After 1 week, mice were euthanized. Regardless of administration route, MSCs from each source yielded: 1) decreased mean linear intercept, neutrophil infiltration, and cell apoptosis; 2) increased elastic fiber content; 3) reduced alveolar epithelial and endothelial cell damage; and 4) decreased keratinocyte-derived chemokine (KC, a mouse analog of interleukin-8) and transforming growth factor-β levels in lung tissue. In contrast with IV, IT MSC administration further reduced alveolar hyperinflation (BM-MSC) and collagen fiber content (BM-MSC and L-MSC). Intravenous administration of BM- and AD-MSCs reduced the number of M1 macrophages and pulmonary hypertension on echocardiography, while increasing vascular endothelial growth factor. Only BM-MSCs (IV > IT) increased the number of M2 macrophages. In conclusion, different MSC sources and administration routes variably reduced elastase-induced lung damage, but IV administration of BM-MSCs resulted in better cardiovascular function and change of the macrophage phenotype from M1 to M2.

【 授权许可】

   
2014 Antunes et al.; licensee BioMed Central Ltd.

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